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Overview

  • Product nameAnti-CENPH antibodySee all CENPH primary antibodies ...
  • Description
    Mouse polyclonal to CENPH
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length CENPH protein (Human)

  • Positive control293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
    • Sequence similaritiesBelongs to the centromere protein H family.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.

      • Target information above from: UniProt accession Q9H3R5 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CENP H antibodyCENP-H antibodyCENPH antibody
          CENPH_HUMAN antibodyCentromere protein H antibodyICEN35 antibodyInterphase centromere complex protein 35 antibodyKinetochore protein CENP H antibodyNNF1 antibodyNNF1, MIND kinetochore complex component, homolog antibodyPMF1 antibody
        see all

      Anti-CENPH antibody images

      • Western blot - CENPH antibody (ab67122)
        Western blot - CENPH antibody (ab67122)
        All lanes : Anti-CENPH antibody (ab67122) at 1/500 dilution

        Lane 1 : CENPH transfected 293T cell lysate
        Lane 2 : Non-transfected 293T cell lysate

        Lysates/proteins at 25 µg/ml per lane.

        Secondary
        Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

        Predicted band size : 28 kDa
        Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      References for Anti-CENPH antibody (ab67122)

      ab67122 has not yet been referenced specifically in any publications.

      Publishing research using ab67122 ? Please let us know so that we can cite the reference in this datasheet

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