Anti-CEP170 antibody (ab84545)
Key features and details
- Rabbit polyclonal to CEP170
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP170 antibody
See all CEP170 primary antibodies -
Description
Rabbit polyclonal to CEP170 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow, Chimpanzee -
Immunogen
Synthetic peptide within Human CEP170 aa 1500 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_055627.2
Database link: Q5SW79 -
Positive control
- HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/500. Permeabilization with Triton-X 100 is recommended.
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Notes |
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ICC/IF
1/100 - 1/500. Permeabilization with Triton-X 100 is recommended. |
Target
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Relevance
CEP170, centrosomal protein 170kDa, plays a role in microtubule organization. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes. -
Cellular localization
Centrosome, centriole. Cytoplasm, cytoskeleton. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis. -
Database links
- Entrez Gene: 9859 Human
- SwissProt: Q5SW79 Human
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Alternative names
- XRCC5 binding protein antibody
- 4933426L22Rik antibody
- A330004A13Rik antibody
see all
Images
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Detection of Human CEP170 by Immunocytochemistry.
Sample: NBF-fixed asynchronous HeLa cells.Antibody: ab84545 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 594 goat anti-rabbit IgG. -
Detection of Human CEP170 by Immunocytochemistry.
Sample: NBF-fixed asynchronous HeLa cells.Antibody: ab84545 used at a dilution of 1/100.
Detection: Red-fluorescent Alexa Fluor 594 goat anti-rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab84545 has been referenced in 3 publications.
- Jerby-Arnon L et al. A Cancer Cell Program Promotes T Cell Exclusion and Resistance to Checkpoint Blockade. Cell 175:984-997.e24 (2018). PubMed: 30388455
- Abdul-Sater Z et al. FANCA safeguards interphase and mitosis during hematopoiesis in vivo. Exp Hematol 43:1031-1046.e12 (2015). IF . PubMed: 26366677
- Hossain M & Stillman B Meier-Gorlin syndrome mutations disrupt an Orc1 CDK inhibitory domain and cause centrosome reduplication. Genes Dev 26:1797-810 (2012). PubMed: 22855792