Overview

  • Product nameAnti-CEP68 antibodySee all CEP68 primary antibodies ...
  • Description
    Rabbit polyclonal to CEP68
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 350-400 (PTNVSPNCPP AEATALPFSG PREPSLKQWP SRVPQKQGGM GLASWSQLAS T) of Human CEP68 (NP_055962.2).

  • Positive control
    • HeLa whole cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was affinity purified using an epitope specific to CEP68 immobilized on a solid support
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab91460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IP
  • Application notesIP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000, or 0.1 - 1µg/ml. Predicted molecular weight: 81 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-CEP68 antibody images

    • ab91460 at 1 µg/ml detecting CEP68 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91460 at 10 µg/mg of lysate (lane 2), CEP68 was also immunoprecipitated by an antibody to an upstream epitope of CEP68 (lane 1) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
    • All lanes : Anti-CEP68 antibody (ab91460) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      developed using the ECL technique

      Predicted band size : 81 kDa


      Exposure time : 3 minutes

    References for Anti-CEP68 antibody (ab91460)

    ab91460 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"