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CETP Activity Assay Kit (Fluorometric) (ab65383)

Q&A (12)

Overview

  • Product nameCETP Activity Assay Kit (Fluorometric)See all Cholesteryl ester transfer protein kits ...
  • Tests
    1 x 100 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: all Mammals
  • Product overview

    Abcam's CETP Activity Assay Kit (Fluorometric) uses a donor molecule containing a fluorescent self-quenched neutral lipid that is transferred to an acceptor molecule in the presence of CETP. CETP-mediated transfer of the fluorescent neutral lipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).
    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCholesteryl ester transfer protein is a member of the lipid transfer/lipopolysaccharide binding protein gene family. Cholesteryl ester transfer protein transfers neutral lipids from high-density lipoprotein (HDL) to very low-density lipoprotein (VLDL) and is present in normal human plasma and serum.
  • Cellular localizationSecreted, extracellular space.
    • Alternative names
        CETPHDLCQ10Lipid transfer proteinLipid transfer protein I

    Applications

    Our Abpromise guarantee covers the use of ab65383 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    CETP Activity Assay Kit (Fluorometric) images

    • Functional Studies - Cholesteryl Ester Transfer Protein Activity Assay Kit (ab65383)
      Functional Studies - Cholesteryl Ester Transfer Protein Activity Assay Kit (ab65383)
      Cholesteryl Ester Transfer Protein Activity Assay with Rabbit.

    Protocols

    References for CETP Activity Assay Kit (Fluorometric) (ab65383)

    ab65383 has not yet been referenced specifically in any publications.

    Publishing research using ab65383 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

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    Displaying 1 - 10 of 12 results for Abreviews and Q&A

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    Inquiry: Could you give me information about the CETP Activity Assay Kit (Fluorometric). On ypur site you recommend to store kit at -20°C. but in the protocol we should see the storage temperature +4°C. What temperature is recommended for the ship...

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    Sorry for the confusion. This kit should be stored at 4 degrees C.

    Customer kindly contacted us regarding the CETP activity assay kit. She was wondering if her frozen human serum samples can be used with the kit. Addtionally, she wanted the recommended sample dilution clarified, since step 4. Note b) mentions diluting to...

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    The laboratory responded that frozen serum samples should be fine to use - as long as they have not undergone multiple freeze-thaw cycles. Additionally, the note of diluting the sample to 500uL with 1x CETP assay buffer is only applicable if a fluoro...

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    Thanks for yor E-mail for help us to solve the problem . The attached is detail of results . below is our answer


    1. Order number ###

    2. Human plasma prepared : the healthy volunteers venous blood was collected into K2EDTA BD-va...

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    Thank you for your reply and for kindly providing these details.

    It has taken some time to discuss this with the originator of the kit, I appreciate your patience.

    Reviewing the details, I suggest to consider the following:
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    Dear Sir /Madam,

    We bought two CETP activity assay kits (ab65383) from your company. Following the instruction we measured a few frozen samples by using a FLX800 Fluorescence Microplate Reader. Excitation 485nm, Emission 535nm and 2ul plasma an...

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    Thank you for taking the time tocontact us and provide these details. I am sorry to hear you have had difficulty obtaining satisfactory results from this kit.

    The details you have kindly provided will enable us to investigate this case for you ...

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    First, with regard to the microplates, should I use black microplates or do transparent ones could also work for the assay?

    Then, with the reaction mix, is it possible to prepare a common mix for all samples (Donor + Acceptor + Buffer + H2O) and ...

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    Thank you for contacting us and for your interest in Abcam products.

    1) Yes, please use black plates for all fluorometric assays.
    2) Yes, you should make a master mix with all components except the samples and distribute it across the wells b...

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    Dear Sir /Madam,

    I have some dyslipidemia samples which were kept at -20oC for 2- 3 years. I hope to measure CETP activity or/and concentration now. Could I ask if CETP activity kit (CETP Activity Assay Kit (Fluorometric) (ab65383)) of your com...

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    Thank you for your enquiry.

    Yes, as long as the samples are frozen appropriately and properly and have not been freeze thawed multiple times, they can be used with ab65383 CETP Activity Assay Kit. Both serum and plasma will make compatible samp...

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    We have some plasma and serum whichhave been stored at -200C for 2-3 years. May I ask if they are suitable to measure CETP activity by using your company CETP activity kit ab65383? Thank you .

    Kind regards

    Thank you for contacting us.

    We have not tried this kit with 2-3 year frozen stored samples however it is most likely to work. In the following publication http://atvb.ahajournals.org/content/15/1/112.full (Fig 2A) the author have observed a litt...

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    Product code: 65383

    Inquiry: I'm interested in studing the intracellular CETP activiy in different cell lines, so I would like to know if our CETP activity assay kit (ab65383) could be used also with cell extract as sample type or only with sup...

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    Thank you for your inquiry.

    I am happy to confirm that ab65383 can be used for cell extracts.

    Please use the assay buffer to prepare the cell extracts.

    I hope this information is helpful and wish you good luck with your resea...

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    Any idea if this kit will work to measure CETP activity in hamster plasma? Thank you

    Thank you for your inquiry.

    This kit works with mammalian plasma samples, soyes it will work with hamster.

    I hope this information helps. Please contact us with any other questions.

    Kunde hat 485nM und 20nM breiter Filter (Excitation), 528nM und 20nM breiter Filter (Emission)

    Vielen Dank für Ihren Anruf.
    Ich habe Rücksprache mit den Fachleuten gehalten und kann Ihnen nun folgendes bestätigen.
    Wenn Ihre Filter (20nM breit) für beide Richtungen sind (+/-), dann können Sie einfach das Kit vermessen ohne Einbußen.
    F...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"