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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-CETP antibody
See all CETP products (5) ...
Description
Rabbit polyclonal to CETP
Specificity
This antibody recognises CETB (70 kDa).
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Immunogen
Synthetic peptide corresponding to the C terminus of Human CETP.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab19012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution (PMID 19287187).
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
Tissue specificity
Expressed by the liver and secreted in plasma.
Involvement in disease
Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP11597
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CETP antibody
- CETP_HUMAN antibody
- Cholesteryl ester transfer antibody
see all
Database links
- Entrez Gene: 1071 Human
- Omim: 118470 Human
- SwissProt: P11597 Human
- SwissProt: P22687 Rabbit
- Unigene: 89538 Human
Anti-CETP antibody images:
Western blot - CETP antibody (ab19012)
Anti-CETP antibody (ab19012)
Predicted band size : 70 kDa
Western blot analysis of CETP expresion in rabbit liver tissue lysate.
Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody (ab19012)
ICC/IF image of ab19012 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19012, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-CETP antibody (ab19012)
This product has been referenced in:
- Cho KHet al. Myocardial infarction patients show altered lipoprotein properties and functions when compared with stable angina pectoris patients. Exp Mol Med 41:67-76 (2009). ELISA; Human.Read more (PubMed: 19287187) »
See 1 publication for this product
Publishing research using ab19012? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"