Anti-CETP antibody (ab51771)

In the coming weeks I am planning to test your ab51771 antibody against CETP. I will test this on both human and mouse liver slides (both paraffin and cryo-slides). I was wondering if you could send me some slides you have tested this antibody on. In this way I can both use your slides as a positive control and have a better look at the morphology of the stained cells and compare them to my own samples.    

ANSWER:

Thank you for contacting us.

We do not have any free samples of the liver tissue that was used to validate this antibody for IHC, but if you are interested in purchasing sections of other liver tissue, we have sections of paraffin-embedded human liver  (ab4348) and mouse liver (ab4607).

Please do not hesitate to contact us if you need any more advice or information.  

 I am planning to do some experiments with your anti-CETP antibody (ab51771). I was wondering if you could send me the protocol you used, or should I be able to get a proper staining on paraffin coupes with the described protocol in the product sheet.  

ANSWER:

Thank you for contacting us.

The conditions for the IHC validation are described briefly in the caption of the image on the online datasheet, which shows a stain of a formalin-fixed, paraffin-embedded human liver section.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

The other details of the protocol are standard. We recommend blocking non-specific staining with a 5% solution of normal serum from the species that the secondary antibody was derived from. The blocking step is typically 30-60 minutes at room temperature, followed by the primary antibody incubation. The wash solutions should contain 0.1% Triton X-100 or Tween-20.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.