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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-CETP antibody
See all CETP products (5) ...
Description
Rabbit polyclonal to CETP
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Rabbit, Hamster, Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human CETP.
(Peptide available as ab71389.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: HeLa, Jurkat, SK N BE, Y79 and HepG2.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-P: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 70 kDa (predicted molecular weight: 55 kDa).
Target
Function
Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
Tissue specificity
Expressed by the liver and secreted in plasma.
Involvement in disease
Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
Cellular localization
Secreted > extracellular space.
Target information above from: UniProt accessionP11597
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CETP antibody
- CETP_HUMAN antibody
- Cholesteryl ester transfer antibody
see all
Database links
- Entrez Gene: 1071 Human
- Omim: 118470 Human
- SwissProt: P11597 Human
- SwissProt: P22687 Rabbit
- Unigene: 89538 Human
Anti-CETP antibody images:
Western blot - CETP antibody (ab51771)
All lanes : Anti-CETP antibody (ab51771) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 55 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
CETP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody (ab51771)
ICC/IF image of ab51771 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51771, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CETP antibody (ab51771)
IHC image of ab51771 staining CETP in Human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-CETP antibody (ab51771)
ab51771 has not yet been referenced specifically in any publications.
Publishing research using ab51771? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"