For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Synthetic peptide designed within the C-terminal region of Human CFTR, conjugated to an immunogenic carrier protein
Our Abpromise guarantee covers the use of ab86190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/2000. Predicted molecular weight: 168 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|IHC-P||1/300 - 1/2000.
Antigen retrieval recommended.
|IHC-Fr||1/300 - 1/2000.|
ab86190 staining CFTR in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2.5% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in TBS + 1% BSA) for 17 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG monoclonal was used as the secondary antibody.
ab86190 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"