Anti-CFTR antibody - C-terminal (ab86190)

Overview

  • Product nameAnti-CFTR antibody - C-terminal
    See all CFTR primary antibodies
  • Description
    Rabbit polyclonal to CFTR - C-terminal
  • Tested applicationsWB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide designed within the C-terminal region of Human CFTR, conjugated to an immunogenic carrier protein

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: Whole serum
  • PurityWhole antiserum
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab86190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/300 - 1/2000. Predicted molecular weight: 168 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
IHC-P 1/300 - 1/2000.

Antigen retrieval recommended.

IHC-Fr 1/300 - 1/2000.

Target

  • FunctionInvolved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
  • Tissue specificityFound on the surface of the epithelial cells that line the lungs and other organs.
  • Involvement in diseaseDefects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
    Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • DomainThe PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
  • Post-translational
    modifications
    Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
    Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling.
  • Cellular localizationEarly endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 35 antibody
    • ABC35 antibody
    • ABCC 7 antibody
    • ABCC7 antibody
    • ATP binding cassette sub family C member 7 antibody
    • ATP Binding Cassette Superfamily C Member 7 antibody
    • ATP binding cassette transporter sub family C member 7 antibody
    • ATP-binding cassette sub-family C member 7 antibody
    • cAMP dependent chloride channel antibody
    • cAMP-dependent chloride channel antibody
    • CF antibody
    • CFTR antibody
    • CFTR/MRP antibody
    • CFTR_HUMAN antibody
    • Channel conductance controlling ATPase antibody
    • Channel conductance-controlling ATPase antibody
    • Cystic fibrosis transmembrane conductance regulator antibody
    • Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody
    • Cystic Fibrosis Transmembrane Regulator antibody
    • dJ760C5.1 antibody
    • MRP 7 antibody
    • MRP7 antibody
    • TNR CFTR antibody
    see all

Anti-CFTR antibody - C-terminal images

  • ab86190 staining CFTR in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 2.5% serum for 20 minutes at room temperature. Samples were incubated with primary antibody (1/1000 in TBS + 1% BSA) for 17 hours at 4°C. An undiluted HRP-conjugated mouse anti-rabbit IgG monoclonal was used as the secondary antibody.

    See Abreview

References for Anti-CFTR antibody - C-terminal (ab86190)

ab86190 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Antigen retrieval step None
Sample Human Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Paraformaldehyde
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Submitted Apr 21 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"