Overview

  • Product nameAnti-CFTR antibody [M3A7]See all CFTR primary antibodies ...
  • Description
    Mouse monoclonal [M3A7] to CFTR
  • SpecificityThis antibody reacts with a protein of 165-170 kDa.
  • Tested applicationsIHC-P, IHC-Fr, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein encoding NBF 2 domain of human CFTR.

  • Positive control
    • Pancreas.
  • General notes


    Cystic Fibrosis Transmembrane Regulator (CFTR) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD) and one R domain. It is structurally similar to multidrug resistance protein (Mdr1). CFTR is found in the apical membranes of epithelia within the pancreas, intestine, bile duct, sweat gland and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Ascites
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notes Cystic Fibrosis Transmembrane Regulator (CFTR) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD) and one R domain. It is structurally similar to multidrug resistance protein (Mdr1). CFTR is found in the apical membranes of epithelia within the pancreas, intestine, bile duct, sweat gland and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
  • Clonality Monoclonal
  • Clone numberM3A7
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionInvolved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
  • Tissue specificityFound on the surface of the epithelial cells that line the lungs and other organs.
  • Involvement in diseaseDefects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
    Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • DomainThe PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
  • Post-translational
    modifications
    Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
    Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling.
  • Cellular localizationEarly endosome membrane.
  • Target information above from: UniProt accession P13569 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ABC 35 antibody
    • ABC35 antibody
    • ABCC 7 antibody
    • ABCC7 antibody
    • ATP binding cassette sub family C member 7 antibody
    • ATP Binding Cassette Superfamily C Member 7 antibody
    • ATP binding cassette transporter sub family C member 7 antibody
    • ATP-binding cassette sub-family C member 7 antibody
    • cAMP dependent chloride channel antibody
    • cAMP-dependent chloride channel antibody
    • CF antibody
    • CFTR antibody
    • CFTR/MRP antibody
    • CFTR_HUMAN antibody
    • Channel conductance controlling ATPase antibody
    • Channel conductance-controlling ATPase antibody
    • Cystic fibrosis transmembrane conductance regulator antibody
    • Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody
    • Cystic Fibrosis Transmembrane Regulator antibody
    • dJ760C5.1 antibody
    • MRP 7 antibody
    • MRP7 antibody
    • TNR CFTR antibody
    see all

Anti-CFTR antibody [M3A7] images

  • ab4067 staining human tonsil by IHC-P.
  • Overlay histogram showing A549 cells stained with ab4067 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4067, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-CFTR antibody [M3A7] (ab4067)

This product has been referenced in:
  • Henderson MJ  et al. Ubiquitin C-terminal hydrolase-L1 protects cystic fibrosis transmembrane conductance regulator from early stages of proteasomal degradation. J Biol Chem 285:11314-25 (2010). WB ; Human . Read more (PubMed: 20147297) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. As we discussed over the phone, this antibody hasn't been tested for cross-reactivity in mouse. Since you are seeing non-specific staining, I would recommend using one of the mouse-on-mouse techniques below with this ant...

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Thanks for your enquiry. This antibody recognizes the C-terminal end of the second nucleotide binding domain (NBF2) of CFTR. If you have any additional questions, please contact us again.

Thank you for your enquiry and your interest in our product. We have optimized this antibody for IHC studies only so it is very difficult to comment on IP or WB protocols. We would suggest trying longer incubation or higher concentration. The concen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"