Overview

  • Product nameAnti-CFTR antibody [MM13-4]See all CFTR primary antibodies ...
  • Description
    Mouse monoclonal [MM13-4] to CFTR
  • Tested applicationsFlow Cyt, WB, IP, ICC, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Pig, Chimpanzee, Baboon, Cynomolgus Monkey, Rhesus monkey, Gorilla

    Does not react with

    Mouse, Shark
  • Immunogen

    Synthetic peptide: RKGYRQRLELSD, corresponding to amino acids 25-36 of Human CFTR

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
  • Concentration information loading...
  • PurityProtein G purified
  • Clonality Monoclonal
  • Clone numberMM13-4
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab77961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 2µg for 106 cells.
WB Use a concentration of 10 µg/ml. Detects a band of approximately 150-170 kDa (predicted molecular weight: 168 kDa).
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionInvolved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
    • Tissue specificityFound on the surface of the epithelial cells that line the lungs and other organs.
    • Involvement in diseaseDefects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
      Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
    • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.
    • DomainThe PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
    • Post-translational
      modifications
      Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
      Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling.
    • Cellular localizationEarly endosome membrane.
    • Target information above from: UniProt accession P13569 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • ABC 35 antibody
      • ABC35 antibody
      • ABCC 7 antibody
      • ABCC7 antibody
      • ATP binding cassette sub family C member 7 antibody
      • ATP Binding Cassette Superfamily C Member 7 antibody
      • ATP binding cassette transporter sub family C member 7 antibody
      • ATP-binding cassette sub-family C member 7 antibody
      • cAMP dependent chloride channel antibody
      • cAMP-dependent chloride channel antibody
      • CF antibody
      • CFTR antibody
      • CFTR/MRP antibody
      • CFTR_HUMAN antibody
      • Channel conductance controlling ATPase antibody
      • Channel conductance-controlling ATPase antibody
      • Cystic fibrosis transmembrane conductance regulator antibody
      • Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody
      • Cystic Fibrosis Transmembrane Regulator antibody
      • dJ760C5.1 antibody
      • MRP 7 antibody
      • MRP7 antibody
      • TNR CFTR antibody
      see all

    Anti-CFTR antibody [MM13-4] images

    • Overlay histogram showing A549 cells stained with ab77961 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77961, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-CFTR antibody [MM13-4] (ab77961)

    This product has been referenced in:
    • Colas J  et al. Disruption of cytokeratin-8 interaction with F508del-CFTR corrects its functional defect. Hum Mol Genet 21:623-34 (2012). IP ; Human . Read more (PubMed: 22038833) »

    See 1 Publication for this product

    Product Wall

    We have not tested the cross-reactivity of ab131553 in mouse however when performing sequence alignments between ab131553's immunogen and the mouse protein, I found 74% homology so it is possible that it may cross-react but we cannot guarantee this. Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"