Overview

  • Product nameAnti-CFTR antibodySee all CFTR primary antibodies ...
  • Description
    Rabbit polyclonal to CFTR
  • SpecificityDetects cystic fibrosis transmembrane conductance factor (CFTR) from cells overexpressing the human protein.
  • Tested applicationsICC, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Monkey, Non Human Primates, Cynomolgus Monkey, Rhesus monkey, Gorilla, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human CFTR aa 103-117.
    Sequence:

    GRIIASYDPDNKEER

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notes Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508) which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is. One possible explanation is that Salmonella typhi has been shown to use CFTR to enter intestinal epithelial cells and that delta F508 heterozygote and homozygote mice showed 86% and 100% reductions in S. typhi intestinal submucosal uptake.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab2916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 168 kDa.

Target

  • FunctionInvolved in the transport of chloride ions. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the SLC4A7 transporter.
  • Tissue specificityFound on the surface of the epithelial cells that line the lungs and other organs.
  • Involvement in diseaseDefects in CFTR are the cause of cystic fibrosis (CF) [MIM:219700]; also known as mucoviscidosis. CF is the most common genetic disease in the Caucasian population, with a prevalence of about 1 in 2'000 live births. Inheritance is autosomal recessive. CF is a common generalized disorder of exocrine gland function which impairs clearance of secretions in a variety of organs. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
    Defects in CFTR are the cause of congenital bilateral absence of the vas deferens (CBAVD) [MIM:277180]. CBAVD is an important cause of sterility in men and could represent an incomplete form of cystic fibrosis, as the majority of men suffering from cystic fibrosis lack the vas deferens.
  • Sequence similaritiesBelongs to the ABC transporter superfamily. ABCC family. CFTR transporter (TC 3.A.1.202) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • DomainThe PDZ-binding motif mediates interactions with GOPC and with the SLC4A7, SLC9A3R1/EBP50 complex.
  • Post-translational
    modifications
    Phosphorylated; activates the channel. It is not clear whether PKC phosphorylation itself activates the channel or permits activation by phosphorylation at PKA sites.
    Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes, enhances its endocytic recycling.
  • Cellular localizationEarly endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC 35 antibody
    • ABC35 antibody
    • ABCC 7 antibody
    • ABCC7 antibody
    • ATP binding cassette sub family C member 7 antibody
    • ATP Binding Cassette Superfamily C Member 7 antibody
    • ATP binding cassette transporter sub family C member 7 antibody
    • ATP-binding cassette sub-family C member 7 antibody
    • cAMP dependent chloride channel antibody
    • cAMP-dependent chloride channel antibody
    • CF antibody
    • CFTR antibody
    • CFTR/MRP antibody
    • CFTR_HUMAN antibody
    • Channel conductance controlling ATPase antibody
    • Channel conductance-controlling ATPase antibody
    • Cystic fibrosis transmembrane conductance regulator antibody
    • Cystic fibrosis transmembrane conductance regulator ATP binding cassette sub family C member 7 antibody
    • Cystic Fibrosis Transmembrane Regulator antibody
    • dJ760C5.1 antibody
    • MRP 7 antibody
    • MRP7 antibody
    • TNR CFTR antibody
    see all

Anti-CFTR antibody images

  • Immunolocalization of CFTR in HEK293 cells stably transfected with the CFTR gene using ab2916.

References for Anti-CFTR antibody (ab2916)

This product has been referenced in:
  • Klein T  et al. Investigation and characterization of the duct cell-enriching process during serum-free suspension and monolayer culture using the human exocrine pancreas fraction. Pancreas 38:36-48 (2009). Read more (PubMed: 18665014) »

See 1 Publication for this product

Product Wall

I'm very sorry to hear one of your customers is experiencing problems with ab2916, indeed CFTR is known to be present in sperm and should be detectable with ab2916. I think the problem comes from the fixation which is damaging the epitopes. I would...

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Thank you for contacting us for some advice on ab2916 and ab2784. You are correct, both antibodies were made using the same immunizing peptide, however they are raised in different species and more importantly we do not know if they recognise the same ...

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Both ab2916 and ab2784 are made from the same synthetic peptide corresponding to amino acid residues 103-117. Ab2784 corresponds to amino acid residues 103-117 found in the first extracellular loop of human and rabbit CFTR. So, it appears that ab...

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I'm sorry to hear you are experiencing problems with ab2916. This antibody has not been tested for western blotting, it is therefore difficult to find out what the problem might be. A litterature search about CFTR enabled me to find that the lysis bu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"