Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

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Abreviews
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Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (DRG neurons)
Specification
DRG neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys

Secondary antibody

Name
Non-Abcam antibody was used: anti - mouse Alexa Fluro 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000

Additional data

Additional Notes
IHC/IF image of ab81887 stained adult DRG neurons. The sections were 4% formaldehyde fixed in 0.3% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab81887) overnight at +4°C. The secondary antibody (red) was Alexa Fluor® 568 donkey anti-mouse used at a 1/1000 dilution for 1h. CGRP expressed in the certain types of neurons mainly in the peri-nuclear location.
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 21 2012

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