Immunohistochemistry (Frozen sections) abreview for Anti-CGRP antibody [4901]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Nervus Suralis peripheral neurons)
Permeabilization
Yes - Triton X-100
Specification
Nervus Suralis peripheral neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Fixative
Acetone

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: PBS+4%FCS+4%NGS+0,4% Triton X-100
Dilution
1/50

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Donkey Anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
Sample: Teased fibers of a biopsy of human Nervus Suralis. Fixation: 10 Minutes in -20°C Acetone
Username

Mr. Thomas Klein

Verified customer

Submitted Sep 11 2015

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