Anti-CHD8 antibody (ab114126)
Key features and details
- Rabbit polyclonal to CHD8
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CHD8 antibody
See all CHD8 primary antibodies -
Description
Rabbit polyclonal to CHD8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 325-350 of Human CHD8 (NP_065971.2)
-
Positive control
- HeLa whole cell lysate (ab150035)
-
General notes
Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99.91% Tris citrate/phosphate
pH: 7-8 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab114126 was affinity purified using an epitope specific to CHD8 immobilized on a solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 291 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 291 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
DNA helicase that acts as a chromatin remodeling factor and regulates transcription. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating to efficient U6 RNA polymerase III transcription. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. CHD8 subfamily.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Post-translational
modificationsSumoylated. -
Cellular localization
Nucleus. Localizes to the promoter regions of several CTNNB1-responsive genes. Also present at known CTCF target sites. - Information by UniProt
-
Database links
- Entrez Gene: 57680 Human
- Entrez Gene: 67772 Mouse
- Omim: 610528 Human
- SwissProt: Q9HCK8 Human
- SwissProt: Q09XV5 Mouse
- Unigene: 530698 Human
- Unigene: 289934 Mouse
-
Alternative names
- ATP dependent helicase CHD8 antibody
- ATP-dependent helicase CHD8 antibody
- Axis duplication inhibitor antibody
see all
Images
-
All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CHD8 with ab114126 at 1/1000 (1µg/ml). Detection: DAB.
-
ab114126 at 3ug/mg of lysate detecting CHD8 by immunoprecipitation (lane 1). 1mg of HeLa whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control.
Detection: chemiluminescence. Exposure time: 30 seconds. -
All lanes : Anti-CHD8 antibody (ab114126) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 291 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
-
Datasheet download
References (6)
ab114126 has been referenced in 6 publications.
- Fan X et al. TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation. Elife 10:N/A (2021). PubMed: 33554859
- Lee CY et al. A spontaneous missense mutation in the chromodomain helicase DNA-binding protein 8 (CHD8) gene: a novel association with congenital myasthenic syndrome. Neuropathol Appl Neurobiol 46:588-601 (2020). PubMed: 32267004
- Zhao C et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell 45:753-768.e8 (2018). PubMed: 29920279
- Wade AA et al. Common CHD8 Genomic Targets Contrast With Model-Specific Transcriptional Impacts of CHD8 Haploinsufficiency. Front Mol Neurosci 11:481 (2018). PubMed: 30692911
- Gompers AL et al. Germline Chd8 haploinsufficiency alters brain development in mouse. Nat Neurosci 20:1062-1073 (2017). WB, ChIP-sequencing ; Mouse . PubMed: 28671691
- Cotney J et al. The autism-associated chromatin modifier CHD8 regulates other autism risk genes during human neurodevelopment. Nat Commun 6:6404 (2015). IP, ChIP . PubMed: 25752243