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Anti-CHFR antibody (ab13773)

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Overview

Product name

Anti-CHFR antibody
See all CHFR products (5) ...

Description

Goat polyclonal to CHFR

Tested applications

ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide: KFNHICEQTRFKN, corresponding to C terminal amino acids 611-623 of Human CHFR.

KFNHICEQTR FKN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

General notes

GenBank Accession Number - NP_060693

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CHFR antibody (ab13773)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CHFR antibody (ab13773) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/64000

    ELISA: 1/64000

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 2.5 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Function

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Tissue specificity

Ubiquitous.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the CHFR family.
Contains 1 FHA domain.
Contains 1 PBZ-type zinc finger.
Contains 1 RING-type zinc finger.

Developmental stage

Weakly expressed in G1 phase, and highly expressed during S phase.

Domain

The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.

Post-translational
modifications

Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
Autoubiquitinated; may regulate its cellular level.
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.

Cellular localization

Nucleus > PML body.

Target information above from: UniProt accessionQ96EP1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Checkpoint with forkhead and ring finger domains antibody
  • Checkpoint with forkhead and RING finger domains protein antibody
  • Chfr antibody
  • CHFR_HUMAN antibody
  • E3 ubiquitin protein ligase CHFR antibody
  • E3 ubiquitin-protein ligase CHFR antibody
  • EC 6.3.2. antibody
  • FLJ10796 antibody
  • FLJ33629 antibody
  • RING finger protein 196 antibody
  • RNF 116 antibody
  • RNF 196 antibody
  • RNF116 antibody
  • RNF196 antibody
  • Ubiquitin ligase protein antibody
see all

Database links

see all

Anti-CHFR antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CHFR antibody (ab13773)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CHFR antibody (ab13773)

ab13773 (2.5 µg/ml) staining CHFR in Human Brain (Cerebellum) by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows stained Purkinje cells in Cerebellum.

References for Anti-CHFR antibody (ab13773)

ab13773 has not yet been referenced specifically in any publications.

Publishing research using ab13773? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"