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- CHFR antibody (ab4184)
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Western blot - CHFR antibody (ab4184)
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Product Name
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CHFR antibody
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See all CHFR antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CHFR
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CHFR. (Peptide available as ab24681.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Specificity
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The immunogen for this antibody is identical in all 3 isoforms of CHFR listed in SwissProt ID Q96EP1.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 73 kDa).
This antibody also appears to work in Immunofluoresence - see picture.
We believe this antibody is probably specific for CHFR because in IF with this antibody we see nuclear dots in SAOS2 cells (which have wt CHFR) but not in U2OS cells (that have a mutation in CHFR and hence low levels of it).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in Hela, Jurkat and A431 whole cell lysates
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.20 - 0.20 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CHFR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CHFR antibody images, references, products related to ab4184 and other tools.
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CHFR antibody images:
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 - CHFR antibody (ab4184)
Immunofluoresence using ab4184 on SAOS2 cells with a FITC conjugated secondary antibody.
The foci seen are confined to the nuclei of the cells present in the picture.
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Western blot - CHFR antibody (ab4184)
All lanes : CHFR antibody (ab4184) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 73 kDa
Observed band size : 76 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
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References for CHFR antibody (ab4184)
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This product has been used in: (two most recent references)
Ahel I et al. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature 451:81-5 (2008). WB; Human. PubMed: 18172500
General references:
If you publish research using ab4184 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CHFR
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CHFR antibody - more information
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Customer reviews (feedback) regarding CHFR antibody |
Customer FAQs regarding CHFR antibody |
Protocols for CHFR antibody |
Price and availability of products related to CHFR antibody |
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CHFR antibody - related products:
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CHFR antibody - other tools:
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Contact Abcam with a Technical Enquiry about CHFR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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