CHFR antibody (ab4184)

Product Name 

CHFR antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to CHFR

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human CHFR.

(Peptide available as ab24681.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms
(Due to sequence homology)

Specificity 

The immunogen for this antibody is identical in all 3 isoforms of CHFR listed in SwissProt ID Q96EP1.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 76 kDa (predicted molecular weight: 73 kDa).

This antibody also appears to work in Immunofluoresence - see picture.

We believe this antibody is probably specific for CHFR because in IF with this antibody we see nuclear dots in SAOS2 cells (which have wt CHFR) but not in U2OS cells (that have a mutation in CHFR and hence low levels of it).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in Hela, Jurkat and A431 whole cell lysates

Cellular localization 

Nuclear

Research areas 

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.20 - 0.20 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

See below for CHFR antibody images, references, products related to ab4184 and other tools.

CHFR antibody images:

References for CHFR antibody (ab4184)

  This product has been used in: (two most recent references)

Ahel I et al. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature 451:81-5 (2008). WB; Human. PubMed: 18172500

General references:
If you publish research using ab4184 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding CHFR antibody

Customer FAQs regarding CHFR antibody

Protocols for CHFR antibody

Price and availability of products related to CHFR antibody

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