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Anti-CHFR antibody (ab57638)

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Overview

Product name

Anti-CHFR antibody
See all CHFR products (5) ...

Description

Mouse monoclonal to CHFR

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length protein, corresponding to amino acids 1-653 of Human CHFR

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Isotype

IgG1

Light chain type

kappa

Research areas

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • IHC-P - CHFR antibody (ab57638)IHC-P - CHFR antibody (ab57638) image (enlarge)

  • Western blot - CHFR antibody (ab57638)Western blot - CHFR antibody (ab57638) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab57638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 1-5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

Tissue specificity

Ubiquitous.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the CHFR family.
Contains 1 FHA domain.
Contains 1 PBZ-type zinc finger.
Contains 1 RING-type zinc finger.

Developmental stage

Weakly expressed in G1 phase, and highly expressed during S phase.

Domain

The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.

Post-translational
modifications

Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
Autoubiquitinated; may regulate its cellular level.
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.

Cellular localization

Nucleus > PML body.

Target information above from: UniProt accessionQ96EP1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Checkpoint with forkhead and ring finger domains antibody
  • Checkpoint with forkhead and RING finger domains protein antibody
  • Chfr antibody
  • CHFR_HUMAN antibody
  • E3 ubiquitin protein ligase CHFR antibody
  • E3 ubiquitin-protein ligase CHFR antibody
  • EC 6.3.2. antibody
  • FLJ10796 antibody
  • FLJ33629 antibody
  • RING finger protein 196 antibody
  • RNF 116 antibody
  • RNF 196 antibody
  • RNF116 antibody
  • RNF196 antibody
  • Ubiquitin ligase protein antibody
see all

Database links

Anti-CHFR antibody images:

  IHC-P - CHFR antibody (ab57638)

IHC-P - CHFR antibody (ab57638)

CHFR antibody (ab57638) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.

  Western blot - CHFR antibody (ab57638)

Western blot - CHFR antibody (ab57638)

Western blot against tagged recombinant protein immunogen using ab57638 CHFR antibody at 1ug/ml. Predicted band size of immunogen is 97 kDa

References for Anti-CHFR antibody (ab57638)

ab57638 has not yet been referenced specifically in any publications.

Publishing research using ab57638? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"