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IHC-P - CHFR antibody (ab57638)
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Western blot - CHFR antibody (ab57638)
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Product Name
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CHFR antibody
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See all CHFR antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to CHFR
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Immunogen
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Recombinant full length protein, corresponding to amino acids 1-653 of Human CHFR
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Reacts with
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Hu
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Tested applications
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
IHC-P: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CHFR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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CHFR antibody images:
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 IHC-P - CHFR antibody (ab57638)
CHFR antibody (ab57638) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.
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Western blot - CHFR antibody (ab57638)
Western blot against tagged recombinant protein immunogen using ab57638 CHFR antibody at 1ug/ml. Predicted band size of immunogen is 97 kDa
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Search PubMed (MEDLINE) for references to CHFR
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CHFR antibody - more information
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Customer reviews (feedback) regarding CHFR antibody |
Customer FAQs regarding CHFR antibody |
Protocols for CHFR antibody |
Price and availability of products related to CHFR antibody |
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CHFR antibody - other tools:
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Contact Abcam with a Technical Enquiry about CHFR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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