CHFR antibody (ab77225)

Product Name 

CHFR antibody

Product type 

Primary antibodies

Description 

Mouse monoclonal to CHFR

Immunogen 

Recombinant fragment: TSGAGAGRGA DPRVPPSSPA TQVCFEEPQP STSTSDLFPT ASASSTEPSP AGRERSSSC, corresponding to amino acids 136-193 of Human CHFR, with a 26kDa tag
BLAST 'TSGAGAGRGA DPRVPPSSPA TQVCFEEPQP STSTSDLFPT ASASSTEPSP ' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

CHFR-transfected 293T cell lysate

Cellular localization 

Nuclear

Research areas 

Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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