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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-CHFR antibody
See all CHFR products (5) ...
Description
Mouse monoclonal to CHFR
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: TSGAGAGRGA DPRVPPSSPA TQVCFEEPQP STSTSDLFPT ASASSTEPSP AGRERSSSC, corresponding to amino acids 136-193 of Human CHFR, with a 26kDa tag
TSGAGAGRGA DPRVPPSSPA TQVCFEEPQP STSTSDLFPT ASASSTEPSP AGRERSSSC
Positive control
CHFR-transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
ELISA: Use at an assay dependent dilution.
Target
Function
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
Tissue specificity
Ubiquitous.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the CHFR family.
Contains 1 FHA domain.
Contains 1 PBZ-type zinc finger.
Contains 1 RING-type zinc finger.
Developmental stage
Weakly expressed in G1 phase, and highly expressed during S phase.
Domain
The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint.
The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M. The FHA domain may be required to interact with phosphorylated proteins.
Post-translational
modifications
Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.
Autoubiquitinated; may regulate its cellular level.
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.
Cellular localization
Nucleus > PML body.
Target information above from: UniProt accessionQ96EP1
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Checkpoint with forkhead and ring finger domains antibody
- Checkpoint with forkhead and RING finger domains protein antibody
- Chfr antibody
see all
Database links
- Clone ID: BC012072 Human
- Entrez Gene: 55743 Human
- IMAGE Id: 4650348 Human
- Omim: 605209 Human
- SwissProt: Q96EP1 Human
- Unigene: 720197 Human
Anti-CHFR antibody images:
Western blot - CHFR antibody (ab77225)
Anti-CHFR antibody (ab77225) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 73 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen was used for the sample.
Western blot - CHFR antibody (ab77225)
All lanes : Anti-CHFR antibody (ab77225) at 5 µg/ml
Lane 1 : CHFR transfected lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 73 kDa
Observed band size : 73 kDa
References for Anti-CHFR antibody (ab77225)
ab77225 has not yet been referenced specifically in any publications.
Publishing research using ab77225? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"