Anti-YKL-40/CHI3L1 antibody (ab180569)
Key features and details
- Rabbit polyclonal to YKL-40/CHI3L1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-YKL-40/CHI3L1 antibody
See all YKL-40/CHI3L1 primary antibodies -
Description
Rabbit polyclonal to YKL-40/CHI3L1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig, Orangutan -
Immunogen
Recombinant full length protein within Human YKL-40/CHI3L1 aa 22-240. The exact sequence is proprietary.
Sequence:MGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFL CTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWN FGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHF TTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDF ISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAP ASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEIC DFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAM VWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT
Database link: P36222 -
Positive control
- WB: MCF-7 and HT-29 cell lysates; IHC: Mouse spleen and liver tissues; ICC/ IF: Raw264.7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 43 kDa.
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IHC-P | (1) |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment. -
Tissue specificity
Present in activated macrophages, articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts. -
Involvement in disease
A genetic variation in CHI3L1 is associated with susceptibility to asthma-related traits type 7 (ASRT7) [MIM:611960]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing and dyspnea, bronchial hyperresponsiveness (BHR) as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 1116 Human
- Entrez Gene: 12654 Mouse
- Omim: 601525 Human
- SwissProt: P36222 Human
- SwissProt: Q61362 Mouse
- Unigene: 382202 Human
- Unigene: 38274 Mouse
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Alternative names
- 39 kDa synovial protein antibody
- ASRT7 antibody
- Cartilage glycoprotein 39 antibody
see all
Images
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Paraffin-embedded mouse spleen tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
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Immunofluorescence staining of Raw264.7 cells stained for YKL-40 with ab180569 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).
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All lanes : Anti-YKL-40/CHI3L1 antibody (ab180569) at 1/1000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : HT-29 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat AntiRabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 43 kDa
Exposure time: 30 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Paraffin-embedded mouse liver tissue stained for YKL-40 using ab180569 at 1/100 dilution in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab180569 has been referenced in 14 publications.
- Wei W et al. Feibi decoction-medicated serum inhibits lipopolysaccharide-induced inflammation in RAW264.7 cells and BMDMs. Exp Ther Med 23:110 (2022). PubMed: 34976152
- Park TI et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat Protoc 17:190-221 (2022). PubMed: 35022619
- Yuan J et al. Adenosine A2A Receptor Suppressed Astrocyte-Mediated Inflammation Through the Inhibition of STAT3/YKL-40 Axis in Mice With Chronic Cerebral Hypoperfusion-induced White Matter Lesions. Front Immunol 13:841290 (2022). PubMed: 35237278
- Lu D et al. Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer. Redox Biol 58:102538 (2022). PubMed: 36417796
- Huan W et al. YKL-40 Aggravates Early-Stage Atherosclerosis by Inhibiting Macrophage Apoptosis in an Aven-dependent Way. Front Cell Dev Biol 9:752773 (2021). PubMed: 34950656