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Overview

  • Product nameAnti-CHKL antibodySee all CHKL primary antibodies ...
  • Description
    Rabbit polyclonal to CHKL
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from an internal region

  • Positive controlHepG2 cell extract A549 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab69934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA ELISA: 1/20000.

Target

  • FunctionHas a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.
  • PathwayPhospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
  • Sequence similaritiesBelongs to the choline/ethanolamine kinase family.
  • Target information above from: UniProt accession Q9Y259 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
      CHETK antibodyChetk-beta antibodyCHKB antibody
      Chkb choline kinase beta antibodyChkb protein antibodyCHKB_HUMAN antibodyCHKL antibodyCholine kinase beta antibodyCholine kinase-like antibodyCholine kinase-like protein antibodyCholine/ethanolamine kinase antibodycholine/ethanolamine kinase beta antibodyCholine/ethanolamine kinase, beta antibodyCK antibodyCK/EK antibodyCK/EK-beta antibodyCKB antibodyCKEKB antibodyEK antibodyEKB antibodyEthanolamine kinase antibodyEthanolamine kinase beta antibodyEthanolamine kinase, beta antibodyMDCMC antibody
    see all

Anti-CHKL antibody images

  • All lanes : Anti-CHKL antibody (ab69934) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : A549 cell extract
    Lane 3 : A549 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 25 kDa,30 kDa,35 kDa,80 kDa. We are unsure as to the identity of these extra bands.

References for Anti-CHKL antibody (ab69934)

ab69934 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"