Overview
- Product nameAnti-CHKL antibodySee all CHKL primary antibodies ...
- DescriptionRabbit polyclonal to CHKL
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide (Human) from an internal region
- Positive controlHepG2 cell extract A549 cell extract
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab69934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
| ELISA | ELISA: 1/20000. |
Target
- FunctionHas a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.
- PathwayPhospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.
- Sequence similaritiesBelongs to the choline/ethanolamine kinase family.
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Database links
- Entrez Gene: 1120 Human
- Omim: 612395 Human
- SwissProt: Q9Y259 Human
- Unigene: 654827 Human
Target information above from: UniProt accession
Q9Y259
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CHETK antibodyChetk-beta antibodyCHKB antibody
- Chkb choline kinase beta antibodyChkb protein antibodyCHKB_HUMAN antibodyCHKL antibodyCholine kinase beta antibodyCholine kinase-like antibodyCholine kinase-like protein antibodyCholine/ethanolamine kinase antibodycholine/ethanolamine kinase beta antibodyCholine/ethanolamine kinase, beta antibodyCK antibodyCK/EK antibodyCK/EK-beta antibodyCKB antibodyCKEKB antibodyEK antibodyEKB antibodyEthanolamine kinase antibodyEthanolamine kinase beta antibodyEthanolamine kinase, beta antibodyMDCMC antibody
see all
Anti-CHKL antibody images
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All lanes : Anti-CHKL antibody (ab69934) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : A549 cell extract
Lane 3 : A549 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 45 kDa
Observed band size : 45 kDa
Additional bands at : 25 kDa,30 kDa,35 kDa,80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-CHKL antibody (ab69934)
ab69934 has not yet been referenced specifically in any publications.
