Overview

  • Product nameAnti-CHMP2A antibodySee all CHMP2A primary antibodies ...
  • Description
    Rabbit polyclonal to CHMP2A
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 169-218 (DELGLSLTDE LSNLPSTGGS LSVAAGGKKA EAAASALADA DADLEERLKN) of Human CHMP2A (NP_055268).

  • Positive control
    • 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab118729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionProbable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release.
  • Sequence similaritiesBelongs to the SNF7 family.
  • DomainThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localizationLate endosome membrane. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • Database links
  • Alternative names
    • BC2 antibody
    • Charged multivesicular body protein 2a antibody
    • CHM2A_HUMAN antibody
    • CHMP2 antibody
    • CHMP2a antibody
    • Chromatin modifying protein 2a antibody
    • Chromatin-modifying protein 2a antibody
    • hVps2 1 antibody
    • hVps2-1 antibody
    • putative breast adenocarcinoma marker antibody
    • Putative breast adenocarcinoma marker BC-2 antibody
    • Putative breast adenocarcinoma marker BC2 antibody
    • Vacuolar protein sorting associated protein 2 1 antibody
    • Vacuolar protein sorting-associated protein 2-1 antibody
    • Vps2 1 antibody
    • VPS2 antibody
    • Vps2-1 antibody
    • VPS2A antibody
    see all

Anti-CHMP2A antibody images

  • Anti-CHMP2A antibody (ab118729) at 1 µg/ml + 293T cell lysate at 10 µg

    Predicted band size : 25 kDa

References for Anti-CHMP2A antibody (ab118729)

ab118729 has not yet been referenced specifically in any publications.

Product Wall

Thank you for supplying us with this extra information about the protocol you used. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

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Looking at the western blot, there is a strong and prominent band after 1 min of exposure using ab118729. This band is at 25kDa on the blot you sent me, is this not the molecular weight that it should be? From my research, I've found that a band a...

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I am sorry to hear that you are experiencing difficulties with one ab118729. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

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