Anti-CHMP4C antibody (ab168205)
Key features and details
- Mouse polyclonal to CHMP4C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CHMP4C antibody -
Description
Mouse polyclonal to CHMP4C -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Chinese hamster -
Immunogen
Recombinant full length protein within Human CHMP4C aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- CHMP4C transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
Target
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Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release. -
Tissue specificity
Expressed in heart, spleen and kidney. -
Sequence similarities
Belongs to the SNF7 family. -
Domain
The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. -
Cellular localization
Cytoplasm > cytosol. Late endosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 92421 Human
- Entrez Gene: 66371 Mouse
- Entrez Gene: 361916 Rat
- Omim: 610899 Human
- SwissProt: Q96CF2 Human
- SwissProt: Q9D7F7 Mouse
- SwissProt: Q569C1 Rat
- Unigene: 183861 Human
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Alternative names
- Charged multivesicular body protein 4c antibody
- CHM4C_HUMAN antibody
- CHMP4c antibody
see all
Images
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All lanes : Anti-CHMP4C antibody (ab168205) at 1 µg/ml
Lane 1 : CHMP4C transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Datasheets and documents
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Datasheet download
References (2)
ab168205 has been referenced in 2 publications.
- Liu B et al. CHMP4C regulates lung squamous carcinogenesis and progression through cell cycle pathway. J Thorac Dis 13:4762-4774 (2021). PubMed: 34527317
- Petsalaki E et al. Chmp4c is required for stable kinetochore-microtubule attachments. Chromosoma N/A:N/A (2018). WB . PubMed: 29968190