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Overview

  • Product nameCHMP6 protein (Tagged-His Tag)See all CHMP6 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 59-201 of Human CHMP6 with an N-terminal His Tag, approximately 28kDa (Uniprot ID = Q96FZ7)
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:reconstitution with 88 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesRAKLLLKKKRYQEQLLDRTENQISSLEAMVQSIEFTQIEM KVMEGLQFGNECLNKMHQ VMSIEEVERILDETQEAVEY QRQIDELLAGSFTQEDEDAILEELSAITQEQIELPEVP SEPLPEKIPENVPVKARPRQAELVAAS
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92042 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Charged multivesicular body protein 6chmp6CHMP6_HUMAN
        Chromatin modifying protein 6Chromatin-modifying protein 6FLJ11749hVps 20hVps20novel SNF7 proteinOTTMUSP00000004363RGD1565325RP24 225K18.4Vacuolar protein sorting associated protein 20Vacuolar protein sorting-associated protein 20Vps 20Vps20
      see all
  • FunctionProbable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SNF7 family.
  • DomainThe acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localizationEndomembrane system. Endosome membrane. Late endosome membrane. Localizes to endosomal membranes.

    • Target information above from: UniProt accession Q96FZ7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CHMP6 protein (Tagged-His Tag) images

    • SDS-PAGE - CHMP6 protein (Tagged-His Tag) (ab92042)
      SDS-PAGE - CHMP6 protein (Tagged-His Tag) (ab92042)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.

    References for CHMP6 protein (Tagged-His Tag) (ab92042)

    ab92042 has not yet been referenced specifically in any publications.

    Publishing research using ab92042 ? Please let us know so that we can cite the reference in this datasheet

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