CHMP6 protein (Tagged-His Tag) (ab92042)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Charged multivesicular body protein 6chmp6CHMP6_HUMAN
- Chromatin modifying protein 6Chromatin-modifying protein 6FLJ11749hVps 20hVps20novel SNF7 proteinOTTMUSP00000004363RGD1565325RP24 225K18.4Vacuolar protein sorting associated protein 20Vacuolar protein sorting-associated protein 20Vps 20Vps20
CHMP6 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. A coloured control band can be seen at 15 kDa (green). The protein-specific band is blue.
References for CHMP6 protein (Tagged-His Tag) (ab92042)
ab92042 has not yet been referenced specifically in any publications.