CIKS 293T Transfected Lysate - (positive control) (ab94245)

Overview

  • Product nameCIKS 293T Transfected Lysate - (positive control)
  • General notesab94245 is a 293T cell transfected lysate in which Human CIKS has been transiently over-expressed using a pCMV-CIKS full length plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. Tissue specificity: Widely expressed. Similarity: Contains 1 SEFIR domain.

Applications

Our Abpromise guarantee covers the use of ab94245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • CIKS 293T Transfected Lysate - (positive control) images

    • ab94245 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-CIKS antibody (ab56758) at 1/500 dilution

      Lane 1 : CIKS 293T Transfected Lysate - (positive control) (ab94245)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      oat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for CIKS 293T Transfected Lysate - (positive control) (ab94245)

    ab94245 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"