Anti-CIKS/ACT1 antibody (ab137395)
Key features and details
- Rabbit polyclonal to CIKS/ACT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CIKS/ACT1 antibody
See all CIKS/ACT1 primary antibodies -
Description
Rabbit polyclonal to CIKS/ACT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human CIKS/ACT1 aa 350-600.
Database link: O43734 -
Positive control
- HepG2 whole cell lysate (ab7900), HeLa cells, Colon ca tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 65 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 65 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 SEFIR domain. - Information by UniProt
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Database links
- Entrez Gene: 10758 Human
- Entrez Gene: 103213 Mouse
- Entrez Gene: 361857 Rat
- Omim: 607043 Human
- SwissProt: O43734 Human
- Unigene: 561514 Human
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Alternative names
- Act 1 antibody
- ACT1 antibody
- Activator of cAMP responsive element modulator (CREM) in testis antibody
see all
Images
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Anti-CIKS/ACT1 antibody (ab137395) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 65 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137395 at 1/200 dilution.
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Immunohistochemical analysis of paraffin embedded Colon ca tissue labelled with ab137395 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab137395 has not yet been referenced specifically in any publications.