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Products:Signal Transduction >> Adapters >> Cytoplasmic
Overview
Product name
Anti-CISH antibody
See all CISH products (2) ...
Description
Rabbit polyclonal to CISH
Tested applications
ELISA, WB, IPmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human
Immunogen
Synthetic peptide, corresponding to amino acids 170-185 of CIS-1
Positive control
Mouse embryo lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This product is raised against Cis-1.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.1 - 1.0 µg/ml.
WB: Use a concentration of 0.5 - 2.0 µg/ml.Detects a band of approximately 47 kDa.
IP: Use 3-5µg for 107 cells.
Target
Function
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificity
Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain.
Post-translational
modifications
Association with EPOR may target the protein for proteolysis by the ubiquitin-dependent proteasome pathway. CIS is mainly monubiquitinated (37 kDa form) but may also exist in a polyubiquitinated form (45 kDa).
Target information above from: UniProt accessionQ9NSE2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- cis 1 antibody
- CIS antibody
- CIS-1 antibody
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Database links
- Entrez Gene: 1154 Human
- Entrez Gene: 12700 Mouse
- Entrez Gene: 83681 Rat
- Omim: 602441 Human
- SwissProt: Q9NSE2 Human
- SwissProt: Q62225 Mouse
- SwissProt: O70512 Rat
- Unigene: 655334 Human
see all
References for Anti-CISH antibody (ab5923)
ab5923 has not yet been referenced specifically in any publications.
Publishing research using ab5923? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"