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Product Name
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CISH antibody
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See all CISH antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to CISH
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Immunogen
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Synthetic peptide, corresponding to amino acids 170-185 of CIS-1
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Reacts with
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Ms
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Predicted to react
(species key)
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Hu, Rat
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IP: Use 3-5µg for 107 cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 47 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse embryo lysate.
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Signal Transduction >> Adapters >> Cytoplasmic
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Relevance
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The eight members of the recently identified Suppressor of Cytokine Signalling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signalling in different ways1. SOCS1 and SOCS6 interact with the insulin receptor (IR) when expressed in human hepatoma cells (HepG2) or in rat hepatoma cells overexpressing human IR. SOCS1 and SOCS6 inhibit insulin-dependent activation of ERK1/2 and protein kinase B in vivo and IR directed phosphorylation of IRS1 in vitro. These results suggest that SOCS proteins may be inhibitors of IR signalling and could mediate cytokine induced insulin resistance and contribute to the pathogenesis of type II diabetes. SOCS6 and SOCS7 are expressed ubiquitously in murine tissues and SOCS6 knockout mice are growth retarded.
This product is raised against Cis-1.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CISH antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to CISH
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CISH antibody - more information
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Customer reviews (feedback) regarding CISH antibody |
Customer FAQs regarding CISH antibody |
Protocols for CISH antibody |
Price and availability of products related to CISH antibody |
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CISH antibody - related products:
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CISH antibody - other tools:
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Contact Abcam with a Technical Enquiry about CISH antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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