Anti-CITED2 antibody (ab184145)
Key features and details
- Rabbit polyclonal to CITED2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CITED2 antibody
See all CITED2 primary antibodies -
Description
Rabbit polyclonal to CITED2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment within Human CITED2 aa 4-191. The exact sequence is proprietary.
Database link: Q99967 -
Positive control
- 293T, HeLa, HepG2 whole cell lysates, Hela cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184145 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 28 kDa.
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ICC/IF | (1) |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 28 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP. -
Sequence similarities
Belongs to the CITED family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10370 Human
- Entrez Gene: 114490 Rat
- Omim: 602937 Human
- SwissProt: Q99967 Human
- Unigene: 82071 Human
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Alternative names
- ASD8 antibody
- Cbp/p300 interacting transactivator 2 antibody
- Cbp/p300 interacting transactivator with Glu/Asp rich carboxy terminal domain 2 antibody
see all
Images
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Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde at room temperature for 15 min labeling CITED2 with ab184145 at 1/1000 (green) and Hoechst 33342 (blue).
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All lanes : Anti-CITED2 antibody (ab184145) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Hela whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 28 kDa12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab184145 has been referenced in 2 publications.
- Zhu H et al. Glucocorticoid counteracts cellular mechanoresponses by LINC01569-dependent glucocorticoid receptor-mediated mRNA decay. Sci Adv 7:N/A (2021). PubMed: 33627425
- Deng Y et al. SOX9 Knockdown-Mediated FOXO3 Downregulation Confers Neuroprotection Against Ischemic Brain Injury. Front Cell Dev Biol 8:555175 (2020). PubMed: 33791290