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CK1 epsilon protein (Tagged-His Tag) (ab91677)

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Overview

  • Product nameCK1 epsilon protein (Tagged-His Tag)See all CK1 epsilon proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 168-416 of Human CK1 epsilon with N terminal His tag. MWt ~31 kDa; UniProt ID = P49674; NCBI accession gi|4503093.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 115 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notes(Amino acid sequence (Sequence determined by 5' Sequencing))
    RENKNLTGTARYASINTHLGIEQSRRDD LESLGYVLMYFNLGSLPWQGLKAATKRQKY ERISEKKM STPIEVLCKGYPSEFSTYLNFCRSLRFDDKPDYSYLRQLF RNLFHRQGFS YDYVFDWNMLKFGAARNPEDVDRERREH EREERMGQLRGSATRALPPGPPTGATAN RLRSAAEPVA STPASRIQPAGNTSPRAISRVDRERKVSMRLHRGAPANVS SSDLTGR QEVSRIPASQTSVPFDHLGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91677 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Casein kinase 1 epsilonCasein kinase ICasein kinase I epsilon
        Casein kinase I isoform epsilonCKI epsilonCKI-epsilonCKIeCKIepsilonCSNK 1ECSNK1EEC 2.7.11.1epsilon isoformHCK IEKC1EKC1E_HUMANkinase CK1 epsilonMGC 10398
      see all
  • FunctionCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. Inhibits cytokine-induced granuloytic differentiation.
  • Tissue specificityExpressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Autophosphorylated.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession P49674 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CK1 epsilon protein (Tagged-His Tag) images

    • SDS-PAGE - CK1 epsilon protein (Tagged-His Tag) (ab91677)
      SDS-PAGE - CK1 epsilon protein (Tagged-His Tag) (ab91677)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CK1 epsilon protein (Tagged-His Tag) (ab91677)

    ab91677 has not yet been referenced specifically in any publications.

    Publishing research using ab91677 ? Please let us know so that we can cite the reference in this datasheet

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