Overview

  • Product nameAnti-CKII alpha antibodySee all CKII alpha primary antibodies ...
  • Description
    Rabbit polyclonal to CKII alpha
  • SpecificityReacts with alpha subunit.
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    The immunogen was a synthetic peptide, which represented a portion of human casein kinase 2, alpha 1 polypeptide encoded within exon 3 (LocusLink ID 1457).

  • Positive control
    • RIPA extract from HeLa cells treated with CKII alpha siRNA or vimentin siRNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab10466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

Anti-CKII alpha antibody images

  • All lanes : Anti-CKII alpha antibody (ab10466) at 1/2500 dilution

    Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 45 kDa
  • All lanes : Anti-CKII alpha antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)

    Lane 1 : Whole cell lysate of HeLa cells
    Lane 2 : siRNA to CK2 alpha in HeLa cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution

    Predicted band size : 45 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Svetlana Khoronenkova

    See Abreview

  • ICC/IF image of ab10466 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10466, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-CKII alpha antibody (ab10466)

This product has been referenced in:
  • Cao JY  et al. Identification of a novel protein interaction motif in the regulatory subunit of casein kinase 2. Mol Cell Biol 34:246-58 (2014). Read more (PubMed: 24216761) »
  • Jarboui MA  et al. Nucleolar Protein Trafficking in Response to HIV-1 Tat: Rewiring the Nucleolus. PLoS One 7:e48702 (2012). WB ; Human . Read more (PubMed: 23166591) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293 cells)
Specification HEK293 cells
Treatment control or CK2 siRNA
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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Verified customer

Submitted May 07 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Formaldehyde
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Submitted Dec 16 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Loading amount 20 µg
Specification Mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
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Submitted Nov 23 2011

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G
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Submitted Jan 18 2011

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 30 µg
Specification HeLa cells
Treatment CK2 alpha siRNA
Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Dr. Svetlana Khoronenkova

Verified customer

Submitted Jul 23 2010

Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 a...

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Ab10466, ab10468 and ab10470 react with the alpha subunit and ab10474 reacts with the alpha prime subunit. Please contact us again if you have any additional questions.

Thank you for your enquiry. The immunogen information for the products are as follows: Ab10466- The immunogen used lies between aa 1-50. Ab10468- The immunogen used lies between aa 300-350. Ab10470- The immunogen used lies between aa350-391. ...

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Thank you for your enquiry and your interest in our product. This particular antibody has only been for Western blot application and we do not have any information if it works for immunocyto/histochemistry. All tested applications are specified...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"