Overview

  • Product nameAnti-CKII alpha antibodySee all CKII alpha primary antibodies ...
  • Description
    Rabbit polyclonal to CKII alpha
  • SpecificityReacts with alpha subunit.
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    The immunogen was a synthetic peptide, which represented a portion of human casein kinase 2, alpha 1 polypeptide encoded within exon 3 (LocusLink ID 1457).

  • Positive control
    • RIPA extract from HeLa cells treated with CKII alpha siRNA or vimentin siRNA.

Properties

Applications

Our Abpromise guarantee covers the use of ab10466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ICC/IF
  • Application notesWB: 1/1000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-CKII alpha antibody images

    • All lanes : Anti-CKII alpha antibody (ab10466) at 1/2500 dilution

      Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size : 45 kDa
    • All lanes : Anti-CKII alpha antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)

      Lane 1 : Whole cell lysate of HeLa cells
      Lane 2 : siRNA to CK2 alpha in HeLa cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution

      Predicted band size : 45 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an Abreview submitted by Svetlana Khoronenkova

      See Abreview

    • ICC/IF image of ab10466 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10466, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-CKII alpha antibody (ab10466)

    This product has been referenced in:
    • Jarboui MA  et al. Nucleolar Protein Trafficking in Response to HIV-1 Tat: Rewiring the Nucleolus. PLoS One 7:e48702 (2012). WB ; Human . Read more (PubMed: 23166591) »
    • Sarkari F  et al. The Herpesvirus Associated Ubiquitin Specific Protease, USP7, Is a Negative Regulator of PML Proteins and PML Nuclear Bodies. PLoS One 6:e16598 (2011). WB ; Human . Read more (PubMed: 21305000) »

    See all 5 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Sample Human Cell (Human primary fibroblasts)
    Specification Human primary fibroblasts
    Permeabilization Yes - 0.2% Triton-X100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Dec 16 2013

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Loading amount 20 µg
    Specification Mouse embryonic fibroblasts
    Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2011

    Application Immunoprecipitation
    Sample Human Cell lysate - whole cell (HeLa cells)
    Total protein in input 200 µg
    Specification HeLa cells
    Immuno-precipitation step Protein A/G
    Username

    Abcam user community

    Verified customer

    Submitted Jan 18 2011

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cells)
    Loading amount 30 µg
    Specification HeLa cells
    Treatment CK2 alpha siRNA
    Gel Running Conditions Reduced Denaturing (10% Tris-Gly gel)
    Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
    Username

    Dr. Svetlana Khoronenkova

    Verified customer

    Submitted Jul 23 2010

    Thank you for your enquiry and patience. Under the conditions tested, which included WB detection at optimized dilutions using lysate from cells expressing endogenous amounts of the subunits, they were specific as specified. That is, ab10466, ab10468 a...

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    Ab10466, ab10468 and ab10470 react with the alpha subunit and ab10474 reacts with the alpha prime subunit. Please contact us again if you have any additional questions.

    Thank you for your enquiry. The immunogen information for the products are as follows: Ab10466- The immunogen used lies between aa 1-50. Ab10468- The immunogen used lies between aa 300-350. Ab10470- The immunogen used lies between aa350-391. ...

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    Thank you for your enquiry and your interest in our product. This particular antibody has only been for Western blot application and we do not have any information if it works for immunocyto/histochemistry. All tested applications are specified...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"