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Product name
CKM Human ELISA Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Human Heart | 4 | 2.66% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Human Heart | 3 | 4.83% |
Tests
1 x 96 well plate
Sample type
Serum, Cell culture extracts, Tissue Extracts
Assay type
Quantitative
Sensitivity
0.245 ng/ml
Range
0.25 ng/ml - 1560 ng/ml
Recovery
85%
Cross reactivity
Reacts with
Human
Notes
ab117993 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human creatine kinase M type (CKM) in serum, cells and tissue lysates. The assay employs an antibody specific for human CKM coated onto well plate strips. Standards and samples are pipetted into the wells and CKM present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- CKM detector antibody is added. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CKM bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Storage: Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Storage: Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 10X Detector Antibody | 1 x 0.7ml | |
| 10X HRP Label | 1 x 1ml | |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Human CKM protein standard (1 µg) | 1 vial | |
| Microplate strips | 1 unit | |
| TMB Development Solution | P/N 8209803 | 1 x 12ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Alternative names
- MS985
Applications
Show applications key
Our Abpromise guarantee covers the use of ab117993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
CKM Human ELISA Kit images:
ELISA - CKM Human ELISA Kit (ab117993)
Example human sample curve. A dilution series of human heart homogenate extract in the working range of the assay.
ELISA - CKM Human ELISA Kit (ab117993)
Example standard curve. A dilution series of purified human CKM in the working range of the assay.
Protocols
References for CKM Human ELISA Kit (ab117993)
ab117993 has not yet been referenced specifically in any publications.
Publishing research using ab117993? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"