Recombinant Anti-CKS2 antibody [EPR7946(2)] (ab155078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7946(2)] to CKS2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CKS2 antibody [EPR7946(2)]
See all CKS2 primary antibodies -
Description
Rabbit monoclonal [EPR7946(2)] to CKS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Drosophila melanogaster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Caco-2, 293T and MOLT4 cell lysates; Human cervical carcinoma and Human kidney tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7946(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. -
Sequence similarities
Belongs to the CKS family. - Information by UniProt
-
Database links
- Entrez Gene: 1164 Human
- Entrez Gene: 66197 Mouse
- Entrez Gene: 498709 Rat
- Omim: 116901 Human
- SwissProt: P33552 Human
- SwissProt: P56390 Mouse
- Unigene: 83758 Human
- Unigene: 222228 Mouse
-
Alternative names
- CDC28 antibody
- CDC28 protein kinase 2 antibody
- CDC28 protein kinase regulatory subunit 2 antibody
see all
Images
-
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CKS2 with ab155078 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling CKS2 with ab155078 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-CKS2 antibody [EPR7946(2)] (ab155078) at 1/1000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 10 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (8)
ab155078 has been referenced in 8 publications.
- Wan Z et al. CKS2 Promotes the Growth in Non-Small-Cell Lung Cancer by Downregulating Cyclin-Dependent Kinase Inhibitor. Pathobiology 89:13-22 (2022). PubMed: 34333494
- Chen M et al. E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastoma. Cell Death Dis 13:784 (2022). PubMed: 36096885
- Lim Y et al. Multiplexed functional genomic analysis of 5' untranslated region mutations across the spectrum of prostate cancer. Nat Commun 12:4217 (2021). PubMed: 34244513
- Hu M et al. CKS2 (CDC28 protein kinase regulatory subunit 2) is a prognostic biomarker in lower grade glioma: a study based on bioinformatic analysis and immunohistochemistry. Bioengineered 12:5996-6009 (2021). PubMed: 34494924
- Liu Y et al. Upregulation of centromere protein M promotes tumorigenesis: A potential predictive target for cancer in humans. Mol Med Rep 22:3922-3934 (2020). PubMed: 33000180
- Deng Y et al. Cyclin-dependent kinase subunit 2 overexpression promotes tumor progression and predicts poor prognosis in uterine leiomyosarcoma. Oncol Lett 18:2845-2852 (2019). PubMed: 31452763
- Li J et al. Integrative analysis of gene expression profiles reveals specific signaling pathways associated with pancreatic duct adenocarcinoma. Cancer Commun (Lond) 38:13 (2018). WB ; Human . PubMed: 29764514
- Yu MH et al. Up-regulated CKS2 promotes tumor progression and predicts a poor prognosis in human colorectal cancer. Am J Cancer Res 5:2708-18 (2015). PubMed: 26609478