Anti-CLASP1 antibody (ab154087)
Key features and details
- Rabbit polyclonal to CLASP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CLASP1 antibody
See all CLASP1 primary antibodies -
Description
Rabbit polyclonal to CLASP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 1-179 of Human CLASP1 (Q7Z460).
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Positive control
- HepG2 and mouse brain whole cell lysates; 4T1 xenograft tissue; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. -
Sequence similarities
Belongs to the CLASP family.
Contains 7 HEAT repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Golgi apparatus > trans-Golgi network. Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2. - Information by UniProt
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Database links
- Entrez Gene: 23332 Human
- Entrez Gene: 76707 Mouse
- Omim: 605852 Human
- SwissProt: Q7Z460 Human
- SwissProt: Q80TV8 Mouse
- Unigene: 469840 Human
- Unigene: 708183 Human
- Unigene: 138740 Mouse
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Alternative names
- 1700030C23Rik antibody
- 5730583A19Rik antibody
- B130045P17Rik antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded 4T1 xenograft tissue labeling CLASP1 with ab154087 at 1/100 dilution.
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Anti-CLASP1 antibody (ab154087) at 1/1000 dilution + Hep G2 whole cell lysate at 30 µg
Predicted band size: 16 kDa
5% SDS PAGE -
Anti-CLASP1 antibody (ab154087) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 16 kDa
5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling CLASP1 with ab154087 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154087 has not yet been referenced specifically in any publications.