Anti-CLEC5A antibody (ab203200)
Key features and details
- Rabbit polyclonal to CLEC5A
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CLEC5A antibody
See all CLEC5A primary antibodies -
Description
Rabbit polyclonal to CLEC5A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Mouse CLEC5A aa 50-150 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9R007 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/20 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
Notes |
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Flow Cyt
1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
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Function
Functions as a positive regulator of osteoclastogenesis. Cell surface receptor that signals via TYROBP. Regulates inflammatory responses. Acts as a key regulator of synovial injury and bone erosion during autoimmune joint inflammation (By similarity). Critical macrophage receptor for dengue virus serotypes 1-4. The binding of dengue virus to CLEC5A triggers signaling through the phosphylation of TYROBP, this interaction does not result in viral entry, but stimulates proinflammatory cytokine release. -
Tissue specificity
Expressed in peripheral blood monocytes and in the monocyte/macrophage cell lines U-937 and Mono-Mac-6, but not in cell lines of other origins. Expression is down-regulated when monocytes differentiate into dendritic cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsN-glycosylated. Contains sialic acid residues. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 23601 Human
- Omim: 604987 Human
- SwissProt: Q9NY25 Human
- Unigene: 446235 Human
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Alternative names
- C type lectin domain family 5 member A antibody
- C-type lectin domain family 5 member A antibody
- C-type lectin superfamily member 5 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon carcinoma tissue labeling CLEC5A with ab203200 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow Cytometric analysis of U937 cells labeling CLEC5A using ab203200 at 1/50 dilution (green) followed by a FITC conjugated secondary, compared to control cells (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab203200 has been referenced in 1 publication.
- Shen J et al. Identification of an Immune-Related Prognostic Gene CLEC5A Based on Immune Microenvironment and Risk Modeling of Ovarian Cancer. Front Cell Dev Biol 9:746932 (2021). PubMed: 34712666