Overview
- Product nameAnti-CLIP170 antibodySee all CLIP170 primary antibodies ...
- DescriptionRabbit polyclonal to CLIP170
- SpecificityThis antibody detects endogenous levels of total CLIP170 protein.
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A synthesized peptide derived from the internal region of human CLIP170.
- Positive controlExtracts from MCF-7 cells, RAW264.7 cells, HepG2 cells, COLO cells and HUVEC cells.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab74240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| WB |
WB: 1/500 - 1/1000. Detects bands of approximately 110 and 161 kDa (predicted molecular weight: 161 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionSeems to be a intermediate filament associated protein that links endocytic vesicles to microtubules.
- Tissue specificityHighly expressed in the Reed-Sternberg cells of Hodgkin disease.
- Sequence similaritiesContains 2 CAP-Gly domains.
- Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules.
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Database links
- Entrez Gene: 6249 Human
- Entrez Gene: 56430 Mouse
- Omim: 179838 Human
- SwissProt: P30622 Human
- SwissProt: Q922J3 Mouse
- Unigene: 524809 Human
- Unigene: 241109 Mouse
- Unigene: 441802 Mouse
Target information above from: UniProt accession
P30622
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CAP GLY domain containing linker protein 1 antibodyCAP-Gly domain-containing linker protein 1 antibodyCLIP 170 antibody
- CLIP antibodyCLIP-170 antibodyCLIP1 antibodyCLIP1_HUMAN antibodyCYLN1 antibodycytoplasmic linker 1 antibodyCytoplasmic linker protein 1 antibodyCytoplasmic linker protein 170 alpha 2 antibodyCytoplasmic linker protein 170 alpha-2 antibodyCytoplasmic linker protein CLIP 170 antibodyReed Steinberg cell expressed intermediate filament associated antibodyReed Sternberg intermediate filament associated protein 3 antibodyReed-Sternberg intermediate filament-associated protein antibodyRestin antibodyRSN antibody
see all
Anti-CLIP170 antibody images
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Western blot - CLIP170 antibody (ab74240)All lanes : Anti-CLIP170 antibody (ab74240) at 1/500 dilution
Lane 1 : Extracts from MCF-7 cells
Lane 2 : Extracts from RAW264.7 cells
Lane 3 : Extracts from HepG2 cells
Lane 4 : Extracts from COLO cells
Lane 5 : Extracts from HUVEC cells
Lane 6 : Extracts from HUVEC cells plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 161 kDa
Observed band size : 161 kDa
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
References for Anti-CLIP170 antibody (ab74240)
ab74240 has not yet been referenced specifically in any publications.
