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Overview

  • Product nameCLNS1A protein (Tagged-His Tag)See all CLNS1A proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 41-237 of Human CLNS1A with N terminal His tag; MWt 39kDa (UniProt P54105).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 148 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesESRLSWLDGSGLGFSLEYPTISLHALSRDRSDCLGEHLYV MVNAKFE EESKEPVADEEEEDSDDDVEPITEFRFVPSD KSALEAMFTAMCECQA LHPDPEDEDSDDYDGEEYDVEA HEQGQGDIPTFYTYEEGLSHLTAEG QATLERLEGMLSQ SVSSQYNMAGVRTEDSIRDYEDGMEVDTTPTVAG QFED ADVDH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91674 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Chloride channelChloride channel nucleotide sensitive 1AChloride channel regulatory protein
        Chloride conductance regulatory protein IClnChloride ion current inducer proteinCLCICLNS 1AClns1aCLNS1BI(Cln)IClnICLN_HUMANMethylosome subunit pIClnnucleotide sensitive 1AReticulocyte pICln
      see all
  • FunctionThe interaction with Sm proteins inhibits their assembly on U RNA and interferes with snRNP biogenesis. Inhibits the binding of survival motor neuron protein (SMN) to Sm proteins. May participate in cellular volume control by activation of a swelling-induced chloride conductance pathway.
  • Sequence similaritiesBelongs to the pICln family.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton. A small fraction is also associated with the cytoskeleton.

    • Target information above from: UniProt accession P54105 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CLNS1A protein (Tagged-His Tag) images

    • SDS-PAGE - CLNS1A protein (Tagged-His Tag) (ab91674)
      SDS-PAGE - CLNS1A protein (Tagged-His Tag) (ab91674)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CLNS1A protein (Tagged-His Tag) (ab91674)

    ab91674 has not yet been referenced specifically in any publications.

    Publishing research using ab91674 ? Please let us know so that we can cite the reference in this datasheet

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