Recombinant HRP Anti-CLPX antibody [EP8772] (ab203694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP8772] to CLPX
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-CLPX antibody [EP8772]
See all CLPX primary antibodies -
Description
HRP Rabbit monoclonal [EP8772] to CLPX -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CLPX. The exact sequence is proprietary.
Database link: O76031 -
Positive control
- WB: HT-1080 and Saos-2 whole cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP8772 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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Notes |
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WB
1/5000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
Target
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Function
ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP. -
Tissue specificity
Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas. -
Sequence similarities
Belongs to the ClpX chaperone family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 10845 Human
- Entrez Gene: 270166 Mouse
- Entrez Gene: 300786 Rat
- SwissProt: O76031 Human
- SwissProt: Q9JHS4 Mouse
- SwissProt: Q5U2U0 Rat
- Unigene: 113823 Human
- Unigene: 30088 Mouse
see all -
Alternative names
- ATP dependent Clp protease ATP binding subunit clpX like, mitochondrial antibody
- ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial antibody
- AU014732 antibody
see all
Images
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All lanes : HRP Anti-CLPX antibody [EP8772] (ab203694) at 1/5000 dilution
Lane 1 : HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate
Lane 2 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203694 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203694 has not yet been referenced specifically in any publications.