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Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
developed using the ECL technique
Predicted band size : 19 kDa
Observed band size : 19 kDa
All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 19 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 6 minutes
Image courtesy of an anonymous Abreview.
All lanes : Anti-CNBP antibody (ab48027) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC-1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 20 µg per lane.
developed using the ECL technique
Predicted band size : 19 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Image courtesy of an anonymous Abreview.
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