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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-CNBP antibody
See all CNBP products (2) ...
Description
Goat polyclonal to CNBP
Specificity
No crossreaction expected with CCHC domain containing 13.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Cynomolgus Monkey, Macaque Monkey
Immunogen
Synthetic peptide: GESGHLARECTIE, corresponding to C terminal amino acids 162-174 of Human CNBP.
GESGHLAREC TIE
Positive control
Human muscle lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
-
Western blot - CNBP antibody (ab48027)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab48027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: 1/8000 (detection limit).
WB: Use at a concentration of 1-3 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
CNBP, also called ZNF9, contains 7 zinc finger domains and is believed to function as an RNA-binding protein. A CCTG expansion in intron 1 of the ZNF9 gene results in myotonic dystrophy type 2. It is also a single stranded DNA-binding protein, with specificity to the sterol regulatory element (SRE). It is involved in sterol-mediated repression.
Cellular localization
Cytoplasmic and Endoplasmic reticulum
Alternative names
- CCHC type zinc finger nucleic acid binding protein antibody
- Cellular nucleic acid binding protein antibody
- CNBP1 antibody
see all
Database links
- Entrez Gene: 395731 Chicken
- Entrez Gene: 504831 Cow
- Entrez Gene: 7555 Human
- Entrez Gene: 12785 Mouse
- Entrez Gene: 64530 Rat
- Entrez Gene: 399294 Xenopus laevis
- NCBI: 4827071 Human
- SwissProt: O42395 Chicken
see all
Anti-CNBP antibody images:
Western blot - CNBP antibody (ab48027)
Anti-CNBP antibody (ab48027) at 2 µg/ml + 35µg Human muscle lysate in RIPA buffer
Secondary
anti-goat-HRP at a 1/3000 dilution
developed using the ECL technique
Predicted band size : 19 kDa
Observed band size : 19 kDa
References for Anti-CNBP antibody (ab48027)
ab48027 has not yet been referenced specifically in any publications.
Publishing research using ab48027? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"