Anti-CNG3 antibody (ab97500)

Overview

• Product nameAnti-CNG3 antibodySee all CNG3 primary antibodies ...
• Description
  Rabbit polyclonal to CNG3
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CNG3.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab109061.)

• Positive controlThis antibody gave a positive signal in Mouse Retina Tissue Lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Sensory System
  • Visual system

  • Neuroscience
  • Sensory System
  • Olfactory system

  • Neuroscience
  • Neurotransmission
  • Receptors / Channels
  • Ligand-Gated Ion Channels
  • More Channels

Applications

Target

• FunctionVisual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.
• Tissue specificityProminently expressed in retina.
• Involvement in diseaseDefects in CNGA3 are the cause of achromatopsia type 2 (ACHM2) [MIM:216900]; also known as total colorblindness or rod monochromacy (RMCH2). ACHM2 is an autosomal recessive condition characterized by day blindness and photophobia. In ACHM2 patients the cones are defective and the subjects see better at night.
• Sequence similaritiesBelongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily.
  Contains 1 cyclic nucleotide-binding domain.
• Cellular localizationMembrane.

Target information above from: UniProt accession Q16281 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1261 Human
  • Omim: 600053 Human
  • SwissProt: Q29441 Cow
  • SwissProt: Q16281 Human
  • Unigene: 234785 Human
  • Unigene: 234785 Human

• Alternative names
  ACHM2 antibodyCCNC1 antibodyCCNCa antibody

  CCNCalpha antibodyCNCG3 antibodyCNG 3 antibodyCNG channel alpha 3 antibodyCNG channel alpha-3 antibodyCNG-3 antibodyCNG3 antibodyCNGA3 antibodyCnga3 cyclic nucleotide gated channel alpha 3 antibodyCNGA3_HUMAN antibodyCone photoreceptor cGMP gated channel antibodyCone photoreceptor cGMP gated channel alpha subunit antibodyCone photoreceptor cGMP gated channel subunit alpha antibodyCone photoreceptor cGMP-gated channel subunit alpha antibodyCyclic nucleotide gated cation channel alpha 3 antibodyCyclic nucleotide gated channel alpha 3 antibodyCyclic nucleotide gated channel olfactory 3 antibodyCyclic nucleotide gated channel rod photoreceptor subunit alpha antibodyCyclic nucleotide-gated cation channel alpha-3 antibodyCyclic nucleotide-gated channel alpha-3 antibodyOTTHUMP00000161120 antibody

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Anti-CNG3 antibody images

• 

  Western blot - CNG3 antibody (ab97500)
  Anti-CNG3 antibody (ab97500) at 1 µg/ml + Mouse Retina Tissue Lysate at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 79 kDa
  Observed band size : 79 kDa
  Additional bands at : 46 kDa,55 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes