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Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab6319 for help.

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3 questions for ab6319

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Question 1

Monday 04-December-2006

What was the protocol for IHC-FR used for this antibody? I am not gettign a signal.

ANSWER:

  

Thank you for your enquiry. I have been in contact with the originator of this antibody and he told me that this antibody was tested for IHC in frozen sections using cryostat or vibratome sections of rat brain. They found nice selective staining of oligodendrocytes in grey and white matter. As for the protocol, he sent me these references.

1.Elisa Barbarese et al., Journal of Cell Science 108, 2781-2790, (1995). 2. LIF receptor signaling limits immune-mediated demyelination by enhancing oligodendrocyte survival Helmut Butzkueven et al. Nature Medicine 8, 613 - 619 (2002). 3. Isobel A. Scarisbrick et al., GLIA 30, 219-230 (2000).

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 2

Friday 03-February-2006

I am very interested in the antibody ab6319. I wonder whether it can recognize the developing myelin or developing oligodendrocytes as ab24566 does for I want to do immunohistochemistry with both newborn and adult mice brain. The 2nd question is whether it can stain the oligodendrocyte's body or not?

ANSWER:

  

Thank you for your enquiry.

I am sorry for the delay to your enquiry.

I am not aware of this antibody been applied to developing myelin or developing oligodendrocytes in NEWBORN mice. Unfortunately this is outside my area of expertise. I also could not find details of CNP1 and CNP2 expression in developing mouse brain in the literature. However, this antibody has been shown to work by IHC-P and IHC-Fr and should you know of the expression of these proteins by these tissues in your mouse system I can see no reason why this antibody would not work.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Question 3

Thursday 25-July-2002

Dear Abcam, I would like to use ab6319 for immunofluorescence in frozen mouse brain sections, but need to have it directly conjugated to Cy3 (or a like fluorophor). Do you offer this service?

ANSWER:

  

Unfortunately, we currently do not offer a conjugation service. However, there are numerous companies advertising their products on the internet who sell "do-it-yourself" conjugation kits.There is no apparent reason why this antibody should not respond well to conjugation.

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