Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Overview

• Product nameAnti-CNPase antibody [11-5B] - Oligodendrocyte MarkerSee all CNPase primary antibodies ...
• Description
  Mouse monoclonal [11-5B] to CNPase - Oligodendrocyte Marker
• SpecificityReacts specifically with CNPase. In an immunoblotting assay, the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands. Immunohistochemical staining of paraffin, cryostat or vibratome sections of rat brain reveals selective staining of oligodendrocytes in the grey and white matter. Nerve cells and axons are not stained and astroglial cells do not appear to be labelled.
• Tested applicationsIHC-FoFr, Flow Cyt, Conjugation, Dot Blot, ELISA, ICC, IHC-P, IHC-Fr, IP, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Dog, Human, Rhesus monkey
  Predicted to work with: Sheep, Rabbit, Cow, Pig

  Does not react with

  Chicken, Guinea pig
• Immunogen

  Full length native protein (purified) (Human).

• Positive controlThis antibody gave a positive signal in Western Blot, when tested against Human, Mouse and Rat Spinal Cord and Brain tissue lysates.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number11-5B
• IsotypeIgG1
• Research Areas
  • Tags & Cell Markers
  • Cell Type Markers
  • Neuroscience Markers
  • Glial

  • Neuroscience
  • Cell Type Marker
  • Glia marker
  • Oligodendrocyte marker

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
• Cellular localizationMembrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P09543 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 280752 Cow
  • Entrez Gene: 607694 Dog
  • Entrez Gene: 1267 Human
  • Entrez Gene: 12799 Mouse
  • Entrez Gene: 493772 Pig
  • Entrez Gene: 25275 Rat
  • Omim: 123830 Human
  • SwissProt: P06623 Cow

  • SwissProt: P09543 Human
  • SwissProt: P16330 Mouse
  • SwissProt: P18104 Pig
  • SwissProt: P13233 Rat
  • SwissProt: P56283 Sheep
  • Unigene: 273621 Human
  • Unigene: 15711 Mouse
  • Unigene: 31762 Rat

  see all

• Alternative names
  2'' antibody2'3' cyclic nucleotide 3' phosphodiesterase antibody2'3'-cyclic-nucleotide 3'-phosphodiesterase antibody

  3''-cyclic-nucleotide 3''-phosphodiesterase antibodyCN37_HUMAN antibodyCNP 1 antibodyCNP antibodyCNP1 antibodyCNPase antibody

  see all

Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker images

• 

  Western blot - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)
  All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/100 dilution
  
  Lane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
  Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 3 : Spinal Cord (Mouse) Tissue Lysate
  Lane 4 : Brain (Mouse) Tissue Lysate
  Lane 5 : Spinal Cord (Rat) Tissue Lysate
  Lane 6 : Brain (Rat) Tissue Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 48 kDa
  
  
  Exposure time : 1 minute
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)Carl Hobbs, King`s College London, United Kingdom
  IHC-P image of CNPase staining on rat brain sections using ab6319 (1:1600). heat mediated antigen retrieval on paraffin embedded sections was performed using citric acid. The sections were then blocked with 1% BSA for 10 min at 21°C. The primary antibody was incubated for 16 hours at 21°C. The sections were then incubated in Goat anti-mouse (Biotin) at 1:200.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

  ab6319 at a 1/2000 dilution staining mouse brain tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2% BSA. The antibody was incubated with the tissue for 10 hours and then bound antibody was detected using a biotinylated goat anti-mouse antibody.

  CNPase staining in the striatum is shown in the image.

  This image is courtesy of an Abreview submitted by Bing Lang on 19 April 2006.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

  ab6319 at a 1/200 dilution staining rat spinal cord cells by ICC/IF. The cells were ethanol fixed and blocked with 5% serum. The antibody was incubated with the cells for 1 hour and then bound antibody was detected using an Alexa Fluor® 488 conjugated Goat anti-mouse antibody.

  This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.

  See Abreview

• 

  Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

  ab6319 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody.

  This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.

  See Abreview

• 

  Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)
  All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/500 dilution
  
  Lane 1 : 40ug rat brain homegenate (whole tissue lysate) animal #661
  Lane 2 : 40ug rat brain homegenate (whole tissue lysate) animal #655
  Lane 3 : 40ug rat brain homegenate (whole tissue lysate) animal #668
  Lane 4 : 40ug rat brain homegenate (whole tissue lysate) animal #662
  Lane 5 : 40ug rat brain homegenate (whole tissue lysate) animal #659
  Lane 6 : 40ug rat brain homegenate (whole tissue lysate) animal #670
  
  Secondary
  HRP conjugated goat anti-mouse antibody
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 48 kDa
  Observed band size : 48 kDa
  
  
  Exposure time : 5 minutes

  This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy

  See Abreview

• 

  Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)
  All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/750 dilution
  
  Lane 1 : Spinal Cord homogenate (whole tissue lysate)
  Lane 2 : Spinal Cord homogenate (whole tissue lysate)
  Lane 3 : Spinal Cord homogenate (whole tissue lysate)
  
  Lysates/proteins at 2 µg per lane.
  
  Secondary
  HRP conjugated sheep anti-mouse IgG
  
  Predicted band size : 48 kDa
  Observed band size : 45,47 kDa (why is the actual band size different from the predicted?)
  

  This image is courtesy of an anonymous Abreview

  See Abreview

• 

  Immunohistochemistry (PFA perfusion fixed frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)This image is courtesy of an anonymous Abreview

  ab6319 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 18 hours at 25°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/250, was used as the secondary.

  Image demonstrates a 2-D depth projection through the superficial cortex.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)Image courtesy of an anonymous Abreview.
  ab6319 staining CNPase in rat oligodendrocytes by Immunocytochemistry/ Immunofluorescence.
  
  Cells were fixed in paraformaldehyde, blocked using 5% serum for 10 minutes at 25°C, then incubated with ab6319 at a 1/200 dilution for 2 hours at 25°C. The secondary used was a goat anti-mouse Cy3 conjugated polyclonal at a 1/100 dilution.

  See Abreview

• 

  Flow Cytometry-Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker(ab6319)
  Overlay histogram showing SH-SY5Y cells stained with ab6319 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6319, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.