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Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

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Overview

Product name

Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker
See all CNPase products (12) ...

Description

Mouse monoclonal [11-5B] to CNPase - Oligodendrocyte Marker

Specificity

Reacts specifically with CNPase. In an immunoblotting assay, the antibody localizes both CNP1 (46kD) and CNP2 (48kD) bands. Immunohistochemical staining of paraffin, cryostat or vibratome sections of rat brain reveals selective staining of oligodendrocytes in the grey and white matter. Nerve cells and axons are not stained and astroglial cells do not appear to be labelled.

Tested applications

IHC-FoFr, Conjugation, Dot Blot, ELISA, ICC, IHC-P, IHC-Fr, IP, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Sheep, Rabbit, Cow, Dog, Pig

Does not react with

Chicken, Guinea pig

Immunogen

Full length native protein (purified) (Human).

Positive control

This antibody gave a positive signal in Western Blot, when tested against Human, Mouse and Rat Spinal Cord and Brain tissue lysates.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

11-5B

Isotype

IgG1

Research areas

Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker

  • Western blot - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)Western blot - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) image (enlarge)

  • Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Cellular localization

Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP09543 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 2'' antibody
  • 2'3' cyclic nucleotide 3' phosphodiesterase antibody
  • 2'3'-cyclic-nucleotide 3'-phosphodiesterase antibody
  • 3''-cyclic-nucleotide 3''-phosphodiesterase antibody
  • CN37_HUMAN antibody
  • CNP 1 antibody
  • CNP antibody
  • CNP1 antibody
  • CNPase antibody
see all

Database links

see all

Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker images:

  Western blot - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Western blot - Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/100 dilution

Lane 1 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)
Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 3 : Spinal Cord (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : Spinal Cord (Rat) Tissue Lysate
Lane 6 : Brain (Rat) Tissue Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 48 kDa


Exposure time : 1 minute

This antibody was raised against full length native CNPase and is predicted to recognize both isoforms. The predicted molecular weights of isoforms CNPI and CNPII are 45- and 48-kDa respectively.

  Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

ab6319 at a 1/2000 dilution staining mouse brain tissue sections by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and blocked with 2% BSA. The antibody was incubated with the tissue for 10 hours and then bound antibody was detected using a biotinylated goat anti-mouse antibody.

CNPase staining in the striatum is shown in the image.

This image is courtesy of an Abreview submitted by Bing Lang on 19 April 2006.

See Abreview

  Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

ab6319 at a 1/200 dilution staining rat spinal cord cells by ICC/IF. The cells were ethanol fixed and blocked with 5% serum. The antibody was incubated with the cells for 1 hour and then bound antibody was detected using an Alexa Fluor® 488 conjugated Goat anti-mouse antibody.

This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.

See Abreview

  Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Immunohistochemistry (Frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

ab6319 at a 1/200 dilution staining rat spinal cord tissue sections from a 4% PFA transcardially perfused animal by Immunohistochemistry (Frozen sections). The tissue was paraformaldehyde fixed and incubated with the antibody for 18 hours. Bound antibody was detected using an HRP conjugated goat anti-mouse polyclonal antibody.

This image is courtesy of an Abreview submitted by Nancy Nutile-McMenemy.

See Abreview

  Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/500 dilution

Lane 1 : 40ug rat brain homegenate (whole tissue lysate) animal #661
Lane 2 : 40ug rat brain homegenate (whole tissue lysate) animal #655
Lane 3 : 40ug rat brain homegenate (whole tissue lysate) animal #668
Lane 4 : 40ug rat brain homegenate (whole tissue lysate) animal #662
Lane 5 : 40ug rat brain homegenate (whole tissue lysate) animal #659
Lane 6 : 40ug rat brain homegenate (whole tissue lysate) animal #670

Secondary
HRP conjugated goat anti-mouse antibody
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 48 kDa
Observed band size : 48 kDa


Exposure time : 5 minutes

This image is courtesy of an Abreview submitted by Ms Nancy Nutile-McMenemy

See Abreview

  Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Western blot - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

All lanes : Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319) at 1/750 dilution

Lane 1 : Spinal Cord homogenate (whole tissue lysate)
Lane 2 : Spinal Cord homogenate (whole tissue lysate)
Lane 3 : Spinal Cord homogenate (whole tissue lysate)

Lysates/proteins at 2 µg per lane.

Secondary
HRP conjugated sheep anti-mouse IgG

Predicted band size : 48 kDa
Observed band size : 45,47 kDa (why is the actual band size different from the predicted?)

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (PFA perfusion fixed frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Immunohistochemistry (PFA perfusion fixed frozen sections) - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

ab6319 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 18 hours at 25°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/250, was used as the secondary.

Image demonstrates a 2-D depth projection through the superficial cortex.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

Immunocytochemistry/ Immunofluorescence - CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

ab6319 staining CNPase in rat oligodendrocytes by Immunocytochemistry/ Immunofluorescence.

Cells were fixed in paraformaldehyde, blocked using 5% serum for 10 minutes at 25°C, then incubated with ab6319 at a 1/200 dilution for 2 hours at 25°C. The secondary used was a goat anti-mouse Cy3 conjugated polyclonal at a 1/100 dilution.

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-CNPase antibody [11-5B] - Oligodendrocyte Marker (ab6319)

This product has been referenced in:

  • Bremer Jet al. Axonal prion protein is required for peripheral myelin maintenance. Nat Neurosci : (2010). WB; Mouse.Read more (PubMed: 20098419) »
  • Luthi-Carter Ret al. SIRT2 inhibition achieves neuroprotection by decreasing sterol biosynthesis. Proc Natl Acad Sci U S A 107:7927-32 (2010). ICC/IF; Mouse.Read more (PubMed: 20378838) »

See all 14 publications for this product

Publishing research using ab6319? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"