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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-CNPase antibody
See all CNPase products (12) ...
Description
Rabbit polyclonal to CNPase
Specificity
ab18527 recognised both isoforms of CNPase.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CNPase.
(Peptide available as ab19423.)
Positive control
This antibody gave a positive signal when tested against Human Brain Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Western blot
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Detects bands of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa). Can be blocked with CNPase peptide (ab19423).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Cellular localization
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP09543
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 2'3'-cyclic-nucleotide 3'-phosphodiesterase antibody
see all
Database links
- Entrez Gene: 280752 Cow
- Entrez Gene: 1267 Human
- Omim: 123830 Human
- SwissProt: P06623 Cow
- SwissProt: P09543 Human
- Unigene: 273621 Human
Anti-CNPase antibody images:
Western blot
All lanes : Anti-CNPase antibody (ab18527) at 1 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Human brain lysate with CNPase peptide (ab19423) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 46, 48 kDa
Observed band sizes: 46 and 48kDa
Expected band sizes: 46 and 48kDa
ab18527 specifically recognises both isoforms of Human CNPase. The activity of the antibody is blocked by the addition of the peptide ab19423.
References for Anti-CNPase antibody (ab18527)
ab18527 has not yet been referenced specifically in any publications.
Publishing research using ab18527? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"