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ab19424 |
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All lanes : Anti-CNPase antibody (ab27695) at 1 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lane 4 : Human brain lysate with
Lane 5 : Mouse brain lysate with
Lane 6 : Rat brain lysate with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 46, 48 kDa
ab27695 recognises both isoforms of CNPase in human brain lysates. In mouse and rat brain lysates the antibody preferentially recognises CNP1 (46kDa). CNP2 (48kDa) is recognised, however the band is significantly weaker. All bands are blocked by the addition of the immunizing peptide, ab19424.
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