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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-CNPase antibody
See all CNPase products (12) ...
Description
Rabbit polyclonal to CNPase
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Rabbit, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CNPase.
(Peptide available as ab19424.)
Positive control
This antibody gave a positive signal when tested against Human, Mouse and Rat Brain Tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Glial
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Western blot
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab27695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 46, 48 kDa (predicted molecular weight: 46, 48 kDa).Can be blocked with CNPase peptide (ab19424).
Target
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Cellular localization
Membrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP09543
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 2'' antibody
- 2'3' cyclic nucleotide 3' phosphodiesterase antibody
- 2'3'-cyclic-nucleotide 3'-phosphodiesterase antibody
see all
Database links
- Entrez Gene: 1267 Human
- Entrez Gene: 12799 Mouse
- Entrez Gene: 25275 Rat
- Omim: 123830 Human
- SwissProt: P09543 Human
- SwissProt: P16330 Mouse
- SwissProt: P13233 Rat
- Unigene: 273621 Human
see all
Anti-CNPase antibody images:
Western blot
All lanes : Anti-CNPase antibody (ab27695) at 1 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lane 4 : Human brain lysate with CNPase peptide (ab19424) at 1 µg/ml
Lane 5 : Mouse brain lysate with CNPase peptide (ab19424) at 1 µg/ml
Lane 6 : Rat brain lysate with CNPase peptide (ab19424) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 46, 48 kDa
ab27695 recognises both isoforms of CNPase in human brain lysates. In mouse and rat brain lysates the antibody preferentially recognises CNP1 (46kDa). CNP2 (48kDa) is recognised, however the band is significantly weaker. All bands are blocked by the addition of the immunizing peptide, ab19424.
References for Anti-CNPase antibody (ab27695)
ab27695 has not yet been referenced specifically in any publications.
Publishing research using ab27695? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"