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Overview

  • Product nameCNPase protein (Tagged-His Tag)See all CNPase proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 4-421 of Human CNPase with N terminal His tag; 418 amino acids, 49kDa (48kDa predicted from sequence). UniProt ID = P09543.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 43 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTV ATLLECKTLFILRGLPG SGKSTLARVIVDKYRDGTKMV SADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIR IL VLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCA QLKEKNQWQLSADDL KKLKPGLEKDFLPLYFGWFLTKK SSETLRKAGQVFLEELGNHKAFKKELRQFVPGDE PREK MDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLK KSYSKAFTLTISA LFVTPKTTGARVELSEQQLQLWPSD VDKLSPTDNLPRGSRAHITLGCAADVEAVQTG LDLLEI LRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVR AIFTGYYGKGK PVPTQGSRKGGALQSCTII
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92238 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        2''2'3' cyclic nucleotide 3' phosphodiesterase3''-cyclic-nucleotide 3''-phosphodiesterase
        CN37_HUMANCNPCNP 1CNP1CNPase
      see all
  • Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
  • Cellular localizationMembrane. Melanosome. Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

    • Target information above from: UniProt accession P09543 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CNPase protein (Tagged-His Tag) images

    • SDS-PAGE - CNPase protein (Tagged-His Tag) (ab92238)
      SDS-PAGE - CNPase protein (Tagged-His Tag) (ab92238)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CNPase protein (Tagged-His Tag) (ab92238)

    ab92238 has not yet been referenced specifically in any publications.

    Publishing research using ab92238 ? Please let us know so that we can cite the reference in this datasheet

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