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Overview

  • Product nameCNTF Human ELISA KitSee all CNTF kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 0.2 ng/ml
  • Range
    0.823 ng/ml - 200 ng/ml
  • Recovery

    > 92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.45 84% - 104%
    Serum 92.35 82% - 102%
    Plasma 93.28 83% - 104%

  • Species reactivity
    Reacts with: Human

    Does not react with

    Rat
  • Product overview

    ab99994 CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTF in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihuman CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • RelevanceCiliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
  • Cellular localizationCytoplasmic
    • Alternative names
        AI429687Ciliary Neurotrophic FactorHCNTF
        MGC41235OTTHUMP00000174731OTTMUSP00000029848
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab99994 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      CNTF Human ELISA Kit images

      • Example of Standard curve for Human CNTF
        Example of Standard curve for Human CNTF

      Protocols

      References for CNTF Human ELISA Kit (ab99994)

      ab99994 has not yet been referenced specifically in any publications.

      Publishing research using ab99994 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab99994 Submit a Question for ab99994

      Displaying 1 - 10 of 10 results for Abreviews and Q&A

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      Inquiry: I have a question regarding your growth factor ELISA kits. I would like to know how the antibodies in these kits are produced. Are they produced in vitro or in vivo (ascities)? Thank you very much!

      Thank you for contacting us.
      Thank you for your inquiry. For the kit ab99994, the capture antibody was collected from mouse ascities after the mouse was injected with the immunogen (so the host is mouse). The host for the detection antibody is goat. ...

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      Will this ELISA kit detect the mouse protein?

      Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins are 9...

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      The 3 ELISA Kits (CNTF ab99994, IGF1 ab100545 and IGFBP2 ab100540) We purchased from your company on Aug 15, 2011 turned out to be quite a disappointment to our research project. While it should work with our human plasma samples as we studied th...

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      I apologize for the delay in getting back to you in regards to this issue, and I appreciate your patience as I have been discussing this situation with my colleagues. I can understand your frustration and disappointment as the results have been unexpected...

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      Here are the data sets you requested. Please let me know if you require anything else.     

      Thank you very much for the data. I am working with some of my colleagues to resolve this as quickly as possible, and I appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

      Hello, Thank you for the response. I would like to clarify that we purchased a total of 8 kits per analyte totalling 24 kits in all. It appears that the refund amount noted is for 1 each of the three kits. Please let me know when to expect the correction....

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      Thank you for your reply. I have found the order with the additional kits that you mentioned. In order to process this refund, I will need a little bit more technical information. Have you tried all of these kits, or did you test 1 from each set and the r...

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      The 3 ELISA Kits (CNTF ab99994, IGF1 ab100545 and IGFBP2 ab100540) We purchased from your company on Aug 15, 2011 turned out to be quite a disappointment to our research project. While it should work with our human plasma samples as we studied th...

      Read More

      Thank you for your reply, and I apologize that these kits did not work well for you. I did not find the attached summary of the test results, could you please send these again? We do fully guarantee these kits to work, so I have issued credit note *** for...

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              OK...I would be willing to try the kit and submit a review for the discount later on...please send the code so that I can order. 

      I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in t...

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      Will either of these CNTF ELISA kits possibly cross react with mouse CNTF?

      Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection ant...

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      I performed 4 times test using your CNTF kit. I got very good signal in my standard each time, but I didn’t get any signal in our healthy control human plasma samples. The plasma sample dilutions I had tested were: undiluted, 3 fold, 9 fold, and 27 fold. ...

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      Thank you for contacting Abcam regarding ab99994. I have received some useful information from the lab regarding the detection of CNTF is plasma samples. CNTF is a fairly low abundant protein in human serum and plasma and therefore may be difficult to...

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      How should I prepare plasma samples for use with this kit? What dilution should be used?

      Thank you for calling Abcam. Regarding the preparation of plasma for use with this kit, please see the following protocol: - Collect blood into EDTA or Sodium heparin tube - Spin 10 minutes at 3000 rpm - Aliquot into small tubes and store at –800...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"