CNTF Rat ELISA Kit (ab100758)
- Product nameCNTF Rat ELISA KitSee all CNTF kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range8.23 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.3 89% - 108% Serum 105.6 93% - 112% Plasma 104.2 92% - 110%
- Species reactivityReacts with: Rat
- Product overview
ab100758 Rat CNTF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CNTF in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Rat CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A animal serum . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml CNTF Microplate (12 strips x 8 wells) coated with anti-Rat CNTF 1 unit CNTF Standard Rat (recombinant) 2 vials Detection Antibody CNTF: biotinylated anti-rat CNTF 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- AI429687Ciliary Neurotrophic FactorHCNTF
Our Abpromise guarantee covers the use of ab100758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for CNTF Rat ELISA Kit (ab100758)
ab100758 has not yet been referenced specifically in any publications.