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Overview

  • Product nameCNTROB protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 483-903 of Human CNTROB With N terminal His tag; 421 amino acids, 55kDa (47kDa predicted from sequence). UniProt ID = Q8N137
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 113 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesKQLQDLSGQHQQELASQLAQFKVEMAEREERQQQVAEDYE LRLAREQ ARVCELQSGNQQLEEQRVELVERLQAMLQAH WDEANQLLSTTLPPPN PPAPPAGPSSPGPQEPEKEERR VWTMPPMAVALKPVLQQSREARDEL PGAPPVLCSSSSD LSLLLGPSFQSQHSFQPLEPKPDLTSSTAGAFSA LGAF HPDHRAERPFPEEDPGPDGEGLLKQGLPPAQLEGLKNFLH QLL ETVPQNNENPSVDLLPPKSGPLTVPSWEEAPQVPR IPPPVHKTKVPL AMASSLFRVPEPPSSHSQGSGPSSGS PERGGDGLTFPRQLMEVSQLL RLYQARGWGALPAEDLL LYLKRLEHSGTDGRGDNVPRRNTDSRLGEI PRKEIPSQ AVPRRLATAPKTEKPPARKKSGHPAPSSMRSRGGVWR
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91887 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        9830165K03RikCentrobincentrobin, centrosomal BRCA2 interacting protein
        Centrosomal BRCA2 interacting proteinCentrosomal BRCA2-interacting proteinCNTRB_HUMANCntrobLIP8LYST interacting protein 8LYST-interacting protein 8PP1221RGD1307488RP23 26L6.9
      see all
  • FunctionRequired for centriole duplication. Inhibition of centriole duplication leading to defects in cytokinesis.
  • Tissue specificityWidely expressed (at protein level). Highly expressed in testis. Also expressed in spleen, thymus, prostate, small intestine, colon and peripheral blood leukocytes.
  • Developmental stagePreferentially incorporated into the newly assembled daughter centriole during centriole assembly at the late G1 or early S phase. Remains in the daughter centrioles throughout the cell cycle. At the next cycle of centriole duplication, its amount on the original daughter centriole eventually decreases.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Centriole-associated, asymmetrically localizes to the daughter centriole.

    • Target information above from: UniProt accession Q8N137 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    CNTROB protein (Tagged-His Tag) images

    • SDS-PAGE - CNTROB protein (Tagged-His Tag) (ab91887)
      SDS-PAGE - CNTROB protein (Tagged-His Tag) (ab91887)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for CNTROB protein (Tagged-His Tag) (ab91887)

    ab91887 has not yet been referenced specifically in any publications.

    Publishing research using ab91887 ? Please let us know so that we can cite the reference in this datasheet

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