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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-EBF1 antibody
See all EBF1 products (4) ...
Description
Rabbit polyclonal to EBF1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Xenopus laevis, Fruit fly (Drosophila melanogaster), Pufferfish (Fugu), Zebrafish
Immunogen
Synthetic peptide derived from within residues 50 - 150 of Human EBF1.
(Peptide available as ab30551.)
Positive control
Ramos (Human Burkitt's lymphoma cell line) and Raji (Human Burkitt's lymphoma cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - EBF1 antibody (ab31735)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
Target
Function
Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Sequence similarities
Belongs to the COE family.
Contains 1 IPT/TIG domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UH73
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COE1 antibody
- OLF1 antibody
- COE1_HUMAN antibody
see all
Database links
- Entrez Gene: 395512 Chicken
- Entrez Gene: 1879 Human
- Entrez Gene: 13591 Mouse
- Entrez Gene: 116543 Rat
- Omim: 164343 Human
- SwissProt: Q9UH73 Human
- SwissProt: Q07802 Mouse
- SwissProt: Q63398 Rat
see all
Anti-EBF1 antibody images:
Western blot - EBF1 antibody (ab31735)
All lanes : Anti-EBF1 antibody (ab31735) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line)
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)
Lysates/proteins at 20 µg per lane.
Secondary
Lane 2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Predicted band size : 66 kDa
Observed band size : 66 kDa
References for Anti-EBF1 antibody (ab31735)
ab31735 has not yet been referenced specifically in any publications.
Publishing research using ab31735? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"