Anti-COL16A1 antibody - C-terminal (ab231044)
Key features and details
- Rabbit polyclonal to COL16A1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COL16A1 antibody - C-terminal -
Description
Rabbit polyclonal to COL16A1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human COL16A1 aa 1400 to the C-terminus (C terminal).
Database link: Q07092-1 -
Positive control
- WB: Recombinant human COL16A1 protein; Human serum. IHC-P: Human lung tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231044 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 158 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 158 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
Involved in mediating cell attachment and inducing integrin-mediated cellular reactions, such as cell spreading and alterations in cell morphology. -
Tissue specificity
In papillary dermis, is a component of specialized fibrillin-1-containing microfibrils, whereas in territorial cartilage matrix, it is localized to a discrete population of thin, weakly banded collagen fibrils in association with other collagens (at protein level). In the placenta, where it is found in the amnion, a membranous tissue lining the amniotic cavity. Within the amnion, it is found in an acellular, relatively dense layer of a complex network of reticular fibers. Also located to a fibroblast layer beneath this dense layer. Exists in tissues in association with other types of collagen. -
Sequence similarities
Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Contains 1 TSP N-terminal (TSPN) domain. -
Developmental stage
Transiently elevated expression during gestation, and decrease at term. -
Domain
This sequence defines eighteen different domains, nine triple-helical domains (COL9 to COL1) and ten non-triple-helical domains (NC10 to NC1). The numerous interruptions in the triple helix may make this molecule either elastic or flexible. -
Post-translational
modificationsProlines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 1307 Human
- Entrez Gene: 107581 Mouse
- Omim: 120326 Human
- SwissProt: Q07092 Human
- SwissProt: Q8BLX7 Mouse
- Unigene: 368921 Human
- Unigene: 41860 Mouse
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Alternative names
- 447AA antibody
- Alpha 1 type XVI collagen antibody
- COGA1_HUMAN antibody
see all
Images
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Formalin-fixed, paraffin-embedded human lung tissue stained for COL16A1 with ab231044 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-COL16A1 antibody - C-terminal (ab231044) at 3 µg/ml + human serum
Predicted band size: 158 kDa -
Anti-COL16A1 antibody - C-terminal (ab231044) at 2 µg/ml + recombinant human COL16A1 protein
Predicted band size: 158 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231044 has not yet been referenced specifically in any publications.